| 摘要: |
| 【 目的】探究唐古特大黄不同外植体诱导愈伤组织分化发育的基因差异表达规律，为优化唐古特大黄 组织培养体系和分子育种奠定基础。【 方法】以唐古特大黄叶片、叶柄及下胚轴为外植体，分别在相同培养基及培养 条件下诱导其愈伤组织，并对不同来源愈伤组织进行转录组测序及基因差异表达分析。【 结果】在相同培养基及培养 条件下，唐古特大黄不同外植体均能诱导形成愈伤组织，诱导率为100%，且不同外植体诱导愈伤组织的生长速度为叶 片>叶柄>下胚轴。但叶片和叶柄诱导的愈伤组织均无法继续分化出不定芽；只有下胚轴诱导的愈伤组织能进一步 分化出不定芽，且诱导率为100%。唐古特大黄不同外植体诱导的愈伤组织在基因表达上存在显著差异，与叶柄诱导 的愈伤组织相比，叶片诱导的愈伤组织有8 970个差异表达基因；与下胚轴诱导的愈伤组织相比，叶片诱导的愈伤组织 有11 956个差异表达基因，叶柄诱导的愈伤组织有7 366个差异表达基因。GO功能富集分析和KEGG通路富集结果 表明，差异表达基因主要显著富集在植物激素信号转导、苯丙烷生物合成及氨基酸代谢等代谢通路中。此外，在唐古特大黄不同外植体诱导的愈伤组织中共鉴定到6 681个基因参与编码57个转录因子家族。RT-qPCR结果表明，PYL4、EIN4、IAA26等基因的表达趋势与转录组学测序结果一致。【 结论】细胞分裂、脱分化与再分化、细胞壁修饰、 激素代谢及转录因子相关基因的差异表达对唐古特大黄愈伤组织的分化具有重要影响。 |
| 关键词: 唐古特大黄 愈伤组织 组织培养 组织分化 基因差异表达 |
| DOI：10. 13207/j. jnwafu. 2026. 07. 017 |
| 分类号: |
| 基金项目:宁夏回族自治区重点研发计划项目（2024BEG02037） |
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| Differential gene expression analysis of callus induced from different explants of Rheum tanguticum |
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YU Wenjing1, YANG Xue1, ZHANG Yingcai1, GAO Lifan1, WANG Lingxia1,2, LIANG Wenyu1,2
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1.College of Life Sciences，Ningxia University，Yinchuan，Ningxia 750021，China;2.National Key Laboratory of Efficient Production of Forest Resources，Yinchuan，Ningxia 750021，China
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| Abstract: |
| 【 Objective】The study aims to explore the differential gene expression patterns during the differentiation and development of callus induced from different explants of Rheum tanguticum，and to lay a found?tion for optimizing its tissue culture system and molecular breeding.【Method】Taking leaves，petioles and hypo? cotyls of R. tanguticum as explants，callus was induced on identical culture medium and under the same condi? tions.Subsequently，transcriptome sequencing and differential gene expression analysis were conducted on callus derived from each explant type.【Result】Under the same culture conditions，different explants of R. tanguticum could induce the formation of callus，with an induction rate of 100%. The callus derived from leaves exhibited the highest growth rate，followed by that from petioles and then hypocotyls. However，the callus induced by leaves and petioles couldn’t continue to differentiate into adventitious buds，only the callus induced by hypocotyls could further differentiate into adventitious buds，with an induction rate of 100%. There were significant differences in gene expression among callus induced from different explants of R. tanguticum.Compared with the callus induced by petioles，the callus induced by leaves had 8 970 differentially expressed genes. Compared with the callus induced by hypocotyls，the callus induced by leaves had 11 956 differentially expressed genes，and the callus induced by petioles had 7 366 differentially expressed genes. The GO functional enrichment analysis and KEGG pathway enrichment results showed that differentially expressed genes were significantly enriched in metabolic pathways including plant hormone signal transduction，phenylpropanoid biosynthesis，and amino acid metabolism. In addition，a total of 6 681 genes were identified to encode 57 transcription factor families in callus induced from different explants of R. tanguticum. The RT-qPCR analysis revealed that the expression patterns of genes including PYL4，EIN4 and IAA26 aligned with the transcriptome sequencing results.【Conclusion】Cell division，dedifferentiation and redifferentiation，cell wall modification，hormone metabolism and the differential expression of genes related to transcription factors have significant influences on the differentiation of R. tanguticum callus. |
| Key words: Rheum tanguticum callus tissue culture tissue differentiation differential gene expression |
