| 摘要: |
| 【目的】挖掘控制玉米体细胞再生的关键基因，为解析植物细胞命运决定的遗传机制，打破体细胞再生的基因型依赖性奠定基础。【方法】以再生能力强的玉米自交系JK11为植物材料，通过对其幼胚进行组织培养，鉴定胚性愈伤诱导率、绿化愈伤率和绿苗再分化率3个体细胞再生相关性状。同时，利用RNA-seq技术对处于4个不同分化时期（0,5,10和15 d）的培养物进行测序，并分别开展比较转录组分析（JK0 vs JK5、JK0 vs JK10和JK0 vs JK15），筛选共同差异表达基因进行功能注释。【结果】自交系JK11的胚性愈伤诱导率、绿化愈伤率和绿苗再分化率的均值分别达到了97.70%，93.91%和86.79%，说明该材料可潜在用于遗传转化。转录组测序共获得12个文库的98.27 Gb高质量数据，平均每个文库的数据量达到了8.19 Gb。比较转录组分析筛选共同差异表达基因3 789个，并划分为6个共表达模块。富集分析结果显示，这些基因主要参与到光合作用、类苯丙烷生物合成、淀粉和蔗糖代谢及植物激素信号转导等通路。此外，蛋白互作网络分析从中鉴定了1个核心节点Zm00001d021784及其得分最高的6个辐射节点，作为控制该性状的关键候选基因，qPCR验证结果显示预测的关键候选基因是可靠的。【结论】筛选体细胞再生中的共同差异表达基因3 789个，其中包括Zm00001d021784在内的7个基因被鉴定为关键候选基因。 |
| 关键词: 玉米 细胞全能性 胚性愈伤组织 组织再生 转录组测序 关键候选基因 |
| DOI：10.13207/j.jnwafu.2026.02.002 |
| 分类号: |
| 基金项目:玉米自交系胚性愈伤组织再生的转录组分析 |
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| Transcriptome analysis of embryogenic callus regeneration in maize inbred line |
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DAI Liqiang1, LI Tianjiao2, QI Yunhong1, WANG Xinling1, ZHANG Jiaqi1, YAO Yanying3, LÜ Wendi3
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1.College of Agronomy,,Jilin Agricultural Science and Technology College,Jilin,Jilin 132101,China;2.College of Biological and Pharmaceutical Engineering,Jilin Agricultural Science and Technology College,Jilin,Jilin 132101,China;3.Jilin Provincial Agricultural Environmental Protection and Rural Energy Management Station,Changchun,Jilin 130033,China
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| Abstract: |
| 【Objective】This study identified key genes controlling somatic regeneration in maize to provide basis for analyzing the genetic mechanism of plant cell fate decision and for breaking the genotype dependence of somatic regeneration.【Method】Maize inbred line JK11 with strong regeneration capacity was selected as the plant material.Three traits related with somatic regeneration,embryogenic callus induction rate (EIR),green callus rate (GCR) and plant regeneration rate (PRR),were identified by tissue culture of its immature embryos.Meanwhile,RNA-seq technology was used to sequence the cultures at four different differentiation time points (0,5,10 and 15 d),and comparative transcriptome analyses were performed separately (JK0 vs JK5，JK0 vs JK10 and JK0 vs JK15).Common differentially expressed genes (DEGs) were screened for functional annotation.【Result】The mean values of EIR,GCR and PRR,reached 97.70%,93.91% and 86.79%,respectively,for the inbred line JK11,indicating that this material had the potential to be used for genetic transformation.After transcriptome sequencing,a total of 98.27 Gb of clean data was obtained to form 12 libraries,with an average of 8.19 Gb per library.After comparative transcriptome analysis,3 789 common DEGs were identified,which were divided into 6 coexpression modules.The enrichment analysis results showed that these genes were mainly involved in the pathways of photosynthesis,phenylpropanoid biosynthesis,starch and sucrose metabolism,and plant hormone signal transduction.Additionally,a hub node Zm00001d021784 and its six radiation nodes with the highest scores were identified by protein-protein interaction network analysis as the key candidate genes controlling this trait.qPCR was used for validation and the results showed that the predicted key candidate genes were reliable.【Conclusion】3 789 genes were screened as common DEGs for somatic regeneration,among which seven genes,including Zm00001d021784,were identified as key candidate genes. |
| Key words: maize cell totipotency embryogenic callus tissue regeneration RNA-seq key candidate genes |
