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内蒙古马铃薯晚疫病菌基因型多样性分析
郭 军1,2, 屈冬玉2, 巩秀峰3
1.西北农林科技大学 植保学院与陕西省农业分子生物学重点实验室;2.中国农科院蔬菜花卉研究所;3.内蒙古正丰马铃薯种业公司
摘要:
为了解和探讨我国内蒙古马铃薯晚疫病菌的基因型多样性,对1997~1999年和2002~2003年采自内蒙古马铃薯主栽区的40个马铃薯晚疫病菌株进行了线粒体DNA单倍型和AFLP分析。结果表明,所有马铃薯晚疫病菌株的线粒体DNA单倍型均为Ⅱa型,说明Ⅱa型可能是目前内蒙古马铃薯晚疫病菌的主要类型,同时病菌群体结构为新的群体结构;所有供试的40个马铃薯晚疫病菌株可划分为17种AFLP基因型,平均每2个菌株为1个特有的基因型,17种基因型分布于3个组中,菌株的亲缘关系与病菌的地理来源、采集年份无相关性。
关键词:  马铃薯晚疫病菌  线粒体DNA  基因型多样性  内蒙古
DOI:
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基金项目:教育部长江学者和创新团队发展计划项目(No. 200558);内蒙古自治区自然科学基金项目(200508010313);中荷联合园艺作物基因组技术实验室资助项目(2001CB711102)
Genotypic diversity in Phytophthora infestans from Inner Mongolia
Abstract:
Forty isolates of Phytophthora infestans collected from Inner Mongolia from 1997 to 1999 and from 2002 to 2003 were analyzed by using mitochondrial DNA halotype and AFLP genotype.All theP.infestans isolates presented mitochondrial DNA haplotype IIa.The results showed that genotypes with the IIa halotype were probably predominant in Inner Mongolia and populations from Inner Mongolia belonged to new populations.17 AFLP genotypes were distinguished among the isolates,in which every second isolate represented an unique genotype.Besides,17 AFLP genotypes were distributed over three clusters.Clustering of AFLP genotypes was not associated with geographical origin or collection year of isolates.
Key words:  Phytophthora infestans  mitochondrial DNA  genotypic diversity  AFLP  Inner Mongolia

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