| 摘要: |
| Ⅰ10菌株是从渤海海水中分离得到的1株放线菌。为了测定Ⅰ10菌珠发酵产物的抗菌活性,以13种病原真菌、3种病原细菌为供试菌,采用抑制菌丝生长速率法和双层平板法,对Ⅰ10菌株发酵液进行了室内杀菌活性测定和盆栽试验,并对其分类地位进行了初步鉴定。结果表明,Ⅰ10菌株发酵液对13种供试病原真菌的菌丝生长抑制率在80％以上的占到76.92％,对3种供试病原细菌的抑菌圈直径达到18 mm以上；Ⅰ10菌株发酵液原液对小麦白粉病的保护和治疗效果分别为99.57％和75.53％。稳定性研究发现,Ⅰ10菌株连续转接10代,其培养特征和产生活性物质性能均表现稳定,第10代菌株发酵液的杀菌活性与出发菌株无明显差异。根据其形态特征、培养特征、生理生化特征,初步鉴定该菌珠为不吸水链霉菌(S.ahygroscopicus)的一个变种。 |
| 关键词: 海洋放线菌 发酵产物 抗菌活性 分类鉴定 不吸水链霉菌 |
| DOI： |
| 分类号: |
| 基金项目:国家“973”项目“从天然生物源小分子发现农用新作用靶标机制及先导结构”（2002AA245121） |
|
| Antibiotic activity of fermentations of marine Actinomycete I10 and initial identification of strain |
|
|
| Abstract: |
| Marine Actinomycete I10 was isolated and collected from seawater of Bohai.The paper invesitagated the antibiotic activity of fermen tatians of stain I10,by means of mycelium growth rate and the double-layer method.The results indicated that more than 80% inhibiting ratio could be gained from the tested 13 varieties of plant pathogenic funguses (76.92% of the total);the diameter of inhibitory ring of 3 tested bacterias was more than 18 mm;the results of pot tests showed that the fermentation products of the strain I10 exhibited 99.57% of protective efficacy and 75.53% of therapeutic efficacy againstPseudoperonospora cubensis.Through the research of stability of strain,the results showed the cultural characteristics and antibiotic productivity of strain I10 were not altered when subcultured 10 generations,and the antibiotic activity of fermentations of the 10th strain did not decrease compared with original strain.According to its morphological,cultural,physi-biochemical characteristics,marine actinomycete I10 was initially identified as a variety of S.ahygroscopicus. |
| Key words: marine Actinomycete,fermentation prlducts,antibiotic activity,identification of strain,S. ahygroscopicus |
