|
|
| |
|
|
码上扫一扫！ |
| 基于转录组与代谢组的黔产红托竹荪子实体 菌裙发育机制解析 |
|
黄晓润1,2,3, 李彪1,2,3, 刘宏宇1,2,3, 黄万兵1,2,3, 刘倾城1,2,3, 郭银萍1,2,3, 龚光禄1,2,3, 杨通静1,2,3, 卢颖颖1,2,3, 尚念杰1,2,3
|
|
1.贵州省农作物品种资源研究所，贵州 贵阳 550000;2.贵州省农业微生物全省重点实验室，贵州 贵阳 550000;3.贵州省食用菌育种重点实验室，贵州 贵阳 550000
|
|
| 摘要: |
| 【 目的】 探究红托竹荪不同生长时期的差异表达基因和差异代谢物及其生物合成途径，为红托竹荪子 实体菌裙生长发育研究奠定基础。【 方法】 以红托竹荪尖顶期（W5_1）、破蛋期（W5_2）和平展期（W5_3）3 个生长时期 的菌裙为研究材料，对红托竹荪不同生长时期的菌裙进行基因组学分析，通过差异倍数（FC）和差异显著水平（P 值）筛 选差异表达基因（DEGs），并对差异表达基因进行 GO 分析和 KEGG 代谢通路分析；同时对其进行代谢组学分析，通过 投影重要性变量（VIP）和差异倍数（FC）筛选差异代谢物，并对差异代谢物进行 KEGG 代谢通路分析。【 结果】 转录组 分析显示，红托竹荪菌裙 W5_2 vs W5_1、W5_3 vs W5_1 和 W5_3 vs W5_2 分别筛选出 3 545，3 664 和 2 024 个差异表 达基因；GO 富集分析显示，各比较组 DEGs 均注释至分子功能、细胞组成和生物过程三大功能类型；KEGG 富集分析 显 示 ，各 比 较 组 DEGs 分 别 显 著 富 集 于 118，118 和 113 条 KEGG 代 谢 通 路 。 代 谢 组 分 析 显 示 ，W5_2 vs W5_1、 W5_3 vs W5_1 和 W5_3 vs W5_2 分别检测出 151，87 和 87 个差异代谢物，KEGG 富集分析显示其分别富集于 75，71 和 71 条 KEGG 代谢通路。对 W5_2 vs W5_1、W5_3 vs W5_1 和 W5_3 vs W5_2 差异代谢物与 DEGs 共同注释的 KEGG 通路进行联合分析，表明 3 个比较组分别显著富集于硫辛酸代谢、淀粉和蔗糖代谢、α-亚麻酸代谢等核心通路。【 结 论】 硫辛酸代谢、淀粉和蔗糖代谢、α-亚麻酸代谢可能是红托竹荪菌裙发育的重要调控通路，初步揭示了红托竹荪菌 裙生长发育的分子调控与代谢规律，为其菌裙发育机制研究及品质调控、栽培优化奠定了理论基础。 |
| 关键词: 红托竹荪 菌裙 差异表达基因 差异代谢物 发育机制 |
| DOI：10. 13207/j. jnwafu. 2027. 01. 016 |
| 分类号: |
| 基金项目:贵州省科技计划项目（黔科合基础-ZK［2023］一般 172）；黔农科院青年基金项目（〔2026〕11 号）；贵州省农业微生物全省重点 实验室项目（黔科合平台［2025］029） |
|
| Integrated transcriptomic and metabolomic analysis on the developmental mechanism for the fruiting body indusium of Dictyophora rubrovolvata from Guizhou |
|
Huang Xiaorun1,2,3, Li Biao1,2,3, Liu Hongyu1,2,3, Huang Wanbing1,2,3, Liu Qingcheng1,2,3, Guo Yinping1,2,3, Gong Guanglu1,2,3, Yang Tongjing1,2,3, Lu Yingying1,2,3, Shang Nianjie1,2,3
|
|
1.Guizhou Institute of Crop Germplasm Resources，Guiyang，Guizhou 550000，China;2.Guizhou Provincial Key Laboratory of Agricultural Microbiology，Guiyang，Guizhou 550000，China;3.Guizhou Key Laboratory of Edible Fungi Breeding，Guiyang，Guizhou 550000，China
|
| Abstract: |
| 【 Objective】 This study aims to explore the differentially expressed genes，differential metabo? lites， and their biosynthetic pathways of Dictyophora rubrovolvata at different growth stages，so as to lay a theo? retical foundation for research on the growth and development of the fruiting body indusium in D. rubrovolvata. 【Method】 Indusium samples of D. rubrovolvata at three key growth stages，namely the apical stage（ W5_1），de? hiscence stage（ W5_2），and flat expansion stage（ W5_3），were selected as research materials. Transcriptomic analysis was performed on these samples at different developmental stages. Differentially expressed genes （DEGs） were screened based on fold change（ FC） and P-value，followed by gene ontology（ GO） annotation and kyoto encyclopedia of genes and genomes（ KEGG） pathway enrichment analysis of the identified DEGs. Meanwhile，metabolomics analysis was conducted. Differential metabolites were screened based on variable im? portance in the projection（ VIP） and FC，after which KEGG pathway enrichment analysis was further per? formed on these metabolites.【Result】 Transcriptomic analysis revealed that 3 545，3 664，and 2 024 DEGs were identified in the comparison groups of W5_2 vs W5_1，W5_3 vs W5_1 and W5_3 vs W5_2，respectively. GO en? richment analysis indicated that the DEGs in each comparison group were annotated into three major functional categories：molecular function，cellular component，and biological process. KEGG enrichment analysis revealed that the DEGs of the three groups were significantly enriched in 118，118，and 113 metabolic pathways，respec? tively. Metabolomic analysis identified 151，87 and 87 differential metabolites in the three comparison groups， which were annotated to 75，71 and 71 KEGG pathways，respectively. A combined analysis was performed on KEGG pathways co-annotated by DEGs and differential metabolites in the three groups. The results demon? strated that the three comparison groups were significantly enriched in core pathways，including lipoic acid me? tabolism，starch and sucrose metabolism，and α-linolenic acid metabolism.【Conclusion】 Lipoic acid metabolism， starch and sucrose metabolism，and α-linolenic acid metabolism may serve as crucial regulatory pathways during indusium development of D. rubrovolvata. This study preliminarily reveals the molecular regulatory and meta? bolic patterns underlying the growth and development of D. rubrovolvata indusium，providing a theoretical basis for further research on its development mechanism，quality regulation，and cultivation optimization. |
| Key words: Dictyophora rubrovolvata indusium differentially expressed genes differential metabolites developmental mechanism |
|
|
|
|
