| 摘要: |
| 【目的】筛选高效纤维素降解菌，并对其产酶条件进行优化，为玉米秸秆等农业废弃物的工业化处理 和利用提供参考。【方法】使用纤维素-刚果红透明圈法，从玉米种植基地腐殖层土壤中分离具有高纤维素酶活性的 降解菌，通过形态学观察并结合分子生物学方法，对筛选出的菌株进行鉴定，然后对该菌株的营养条件和培养条件进 行优化。【结果】从土壤中分离获得一株纤维素降解菌ZZ04，经鉴定为贝莱斯芽孢杆菌（Bacillus velezensis），该菌株培养时的最适碳源为羧甲基纤维素钠（CMC-Na），氮源为蛋白胨与酵母粉的等量混合物，碳氮比为2∶4，接种量为3%，培养基初始pH值为7，培养温度为35 ℃，培养时间为48 h，在此条件下菌株ZZ04产羧甲基纤维素酶（CMC）活性和滤纸酶（FPA）活性分别高达222.14和171.21 U/mL。【结论】筛选的贝莱斯芽孢杆菌ZZ04在最佳培养条件下的纤维素 酶活性较高，具有开发微生物降解菌剂的潜力。 |
| 关键词: 纤维素降解菌 贝莱斯芽孢杆菌 微生物降解菌剂 营养条件 培养条件 纤维素酶活性 |
| DOI：10. 13207/j. jnwafu. 2026. 12. 015 |
| 分类号: |
| 基金项目:福建省属公益类科研院所专项（2023R1022004，2023R1071）；福建省农业高质量发展超越“5511”协同创新工程项目 （XTCXGC2021011，XTCXGC2021017） |
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| Isolation，identification，and cellulase production optimization of cellulolytic bacterium ZZ04 |
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HUANG Peng1, HOU Xiangyu2,3,4, ZHANG Jie2, LI Benjin2, YAO Jinai2, YU Deyi2
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1.Institute of Plant Protection，Fujian Academy of Agricultural Sciences Fujian/Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fujian Engineering Research Center for Green Pest Management，Fuzhou，Fujian 350013;2.Institute of Plant Protection，Fujian Academy of Agricultural Sciences Fujian/Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fujian Engineering Research Center for Green Pest Management，Fuzhou，Fujian 350013，China;3.China;4.Fuzhou Institute of Technology，Fuzhou，Fujian 350506，China
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| Abstract: |
| 【Objective】This study aims to screen for efficient cellulose-degrading strains，and optimizes their enzyme production condition，thereby providing a reference for the industrial treatment and utilization of such agricultural waste as corn straw.【Method】Using the cellulose-Congo red clear zone method，strains with high cellulase activity were isolated from the humus layer soil of a corn planting base. The selected strains were identified by morphological observation combined with molecular biological analysis，followed by optimization of their nutritional and cultural conditions.【Result】A cellulose-degrading strain ZZ04 was isolated from soil and identified as Bacillus velezensis. The optimal culture conditions for this strain were as follows：CMC-Na as the carbon source，an equal mixture of peptone and yeast extract as the nitrogen source，a carbon-to-nitrogen ratio of 2∶4，an inoculation size of 3%，an initial medium pH of 7，a culture temperature of 35 ℃，and an incubation time of 48 h. Under these conditions，the carboxymethyl cellulase（CMC） activity and filter paper activity（FPA） of the strain reached 222.14 U/mL and 171.21 U/mL，respectively.【Conclusion】The screened strain B.velezensis ZZ04 exhibits high cellulase activity under optimal culture conditions，indicating its potential for development as a microbial degradation agent. |
| Key words: cellulose-degrading bacterium Bacillus velezensis microbial degradation agent nutritional conditions culture conditions cellulase activity |
