码上扫一扫！ |
| 兴安落叶松苗期抗旱性评价体系的建立 |
|
胡龙胜,马文旭,杨春会,燕帅旭,余合惠子,贾辰琳,王福德,邓澍荣,罗志斌
|
|
1.中国林业科学研究院 林业研究所 林木遗传育种全国重点实验室，北京 100091;2.国家林业和草原局盐碱地中心，北京 100091;3.黑龙江省林业科学研究所，黑龙江 哈尔滨 150080;4.中国林业科学研究院 生态保护与修复研究所，北京，100091;5.中国林业科学研究院 黄河三角洲综合试验中心，山东 东营，257000
|
|
| 摘要: |
| 【 目的】建立兴安落叶松（Larix gmelinii）种质资源抗旱性评价体系。【 方法】以兴安落叶松两年生盆 栽苗为材料，进行中度和重度干旱胁迫处理，记录不同程度干旱胁迫下的损伤表型并进行分级，测定光合参数、渗透调 节物含量和抗氧化酶活性等生理生化指标，利用主成分分析法和隶属函数法计算综合抗旱系数（CDC）、综合抗旱指数 （CDI）、加权抗旱性系数（WDC）、抗旱性综合度量值（D），用Logistic回归模型进行分析。【 结果】在中度和重度干旱 胁迫下，兴安落叶松苗的生物量受到抑制，植株的损伤程度可分为5个等级。干旱胁迫处理兴安落叶松苗的甜菜碱、 类胡萝卜素、叶绿素a、过氧化氢、丙二醛含量及气孔导度、针叶相对电导率、针叶温度、净光合速率、针叶相对含水量和 蒸腾速率等11个生理指标发生显著性（P<0.05）变化。构建了4个抗旱性评价模型，其中以叶绿素a、类胡萝卜素含 量、蒸腾速率、针叶温度、净光合速率为关键指标构建的抗旱性综合度量值（D）模型与植株胁迫损伤表型等级相关性 最高；Logistic回归模型分析结果表明，中度和重度干旱胁迫下的耐旱阈值（DT50）分别为0.505和0.444。【 结论】抗旱性综合度量值是幼苗期兴安落叶松最适宜的抗旱性评价模型，叶绿素a、类胡萝卜素含量、蒸腾速率、净光合速率、针叶 温度可作为抗旱性评价的关键指标。中度、重度干旱胁迫下的D值分别大于0.505或0.444的兴安落叶松幼苗耐旱性强。 |
| 关键词: 兴安落叶松 干旱胁迫 抗旱性综合评价 |
| DOI：10. 13207/j. jnwafu. 2026. 11. 005 |
| 分类号: |
| 基金项目:农业生物育种国家科技重大专项（2023ZD04058）；中央级公益性科研院所基本科研业务费专项资金项目（CAFYBB2024QF009） |
|
| Establishment of a drought resistance evaluation system of Larix gmelinii seedlings |
|
HU Longsheng1, MA Wenxu1, YANG Chunhui2, YAN Shuaixu1, YU Hehuizi2, JIA Chenlin3, WANG Fude4, DENG Shurong1,5,6, LUO Zhibin1,5,6
|
|
1.State Key Laboratory of Tree Genetics and Breeding，Research Institute of Forestry，Chinese Academy of Forestry，Beijing 100091，China;2.Research Center of Saline and Alkali Land of State Forestry Administration，Beijing 100091，China;3.State Key Laboratory of Tree Genetics and Breeding，Research Institute of Forestry，Chinese Academy of Forestry，Beijing 100091，China1，China;4.Heilongjiang Academy of Forestry，Harbin，Heilongjiang 150080，China;5.Institute of Ecological Conservation and Restoration，Chinese Academy of Forestry，Beijing 100091，China;6.Comprehensive Experimental Center in Yellow River Delta，Chinese Academy of Forestry，Dongying，Shandong 257000，China
|
| Abstract: |
| 【 Objective】This study aims to establish a comprehensive drought resistance evaluation system for Larix gmelinii germplasm resources.【Method】Using two-year-old potted seedlings of Larix gmelinii as ex?perimental material，treatments of moderate drought stress and severe drought stress were conducted，and phenotypic damage under varying degrees of drought stress was recorded and classified.Physiological and biochemical parameters，including photosynthetic characteristics，osmoregulatory substances，and antioxidant enzyme activities，were measured. Principal component analysis and membership function methods were employed to calcu-late the following indices：comprehensive drought resistance coefficient（CDC），comprehensive drought resis-tance index（CDI），weighted drought resistance coefficient（WDC），comprehensive drought resistance evalua-tion value（D），and Logistic regression model was used for analysis.【Result】Under different drought stress levels，the growth of Larix gmelinii seedlings were inhibited，and the phenotypic damage was classified into five lev-els. Under moderate and severe drought conditions，significant difference was observed in the values of 11 physi-ological indicators（P<0.05），including betaine content，carotenoids content，chlorophyll a content，stomatal conductance，hydrogen peroxide content，relative electrical conductivity of needles，needle temperature，malondi-aldehyde content，net photosynthetic rate，relative water content of needles，and transpiration rate，in comparison with those under the control.A comprehensive drought resistance evaluation value（D） model，which was con-structed using the key indicators including chlorophyll a content，carotenoid content，transpiration rate，needle temperature， and net photosynthetic rate，exhibited the highest consistency with phenotypic damage levels，indi-cating it is the most suitable model for evaluating drought resistance of Larix gmelinii seedlings. The results of the Logistic regression analysis revealed that the drought tolerance thresholds（DT50） under moderate and se?vere drought stress were 0.505 and 0.444，respectively.【Conclusion】The comprehensive drought resistance evaluation value model is the optimal method for evaluating drought resistance of Larix gmelinii seedlings. Contents of chlorophyll a and carotenoids，transpiration rate，net photosynthetic rate，and needle temperature can serve as key indicators for drought resistance evaluation.And Larix gmelinii seedlings with a D-value greater than 0.505 under moderate drought stress or greater than 0.444 under severe drought stress exhibit strong drought tolerance. |
| Key words: Larix gmelinii drought stress comprehensive drought resistance evaluation |
