| 摘要: |
| 【 目的】探究外源多胺（精胺、亚精胺）对高温胁迫下百合花粉萌发的调控作用。【 方法】以新鲜麝香百 合花粉为材料，优化离体培养条件后设置25，30，35和40 ℃温度处理，通过测定不同温度处理下百合花粉的萌发率和 花粉管长度，筛选后续高温胁迫温度。在25 ℃（对照）和35 ℃（高温胁迫）培养条件下，向培养液中分别添加0（对照），0.025，0.05和0.1 mmol/L 的精胺（Spd）或亚精胺（Spm），检测不同处理百合花粉的萌发率、花粉管长度、畸变率、活力、活性氧分布及胼胝质分布情况。【 结果】①百合花粉离体培养最适液体培养基配方（质量分数）：蔗糖10%，硼酸0.06%，氯化钙0.02%，硫酸镁0.02%，磷酸二氢钾0.02%。②与对照（25 ℃）相比，高温胁迫显著抑制了百合花粉萌 发和花粉管生长，导致花粉管出现破裂、弯曲及顶端膨大等异常形态。③在 35 ℃高温胁迫下，与对照相比，添加0.05 mmol/L Spd使百合花粉萌发率和花粉管长度显著增加了24.78%和16.36%；添加0.025 mmol/L Spm使百合花粉萌 发率提高了7.22%，但明显抑制了花粉管伸长；添加0.05 mmol/L Spd或0.025 mmol/L Spm分别使百合花粉管畸变 率降低了21.65%和26.69%，有效缓解了高温引起的活性氧分布异常及胼胝质沉积紊乱，提高了花粉管的活力。【 结 论】高温胁迫显著抑制了百合花粉萌发和花粉管生长，并导致花粉管畸形。添加0.05 mmol/L Spd能有效恢复高温胁 迫下百合花粉萌发和花粉管正常伸长，但Spm仅对花粉萌发有促进作用。外源多胺主要通过恢复花粉管内活性氧信 号和胼胝质分布来提高花粉管活力，从而显著缓解高温损伤。 |
| 关键词: 麝香百合 高温胁迫 花粉萌发 花粉管伸长 花粉管形态 活性氧 胼胝质沉积 |
| DOI：10. 13207/j. jnwafu. 2026. 09. 016 |
| 分类号: |
| 基金项目:国家自然科学基金项目（31301733）；山东省大学生创新创业训练计划项目（S202511067034） |
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| Regulation of exogenous polyamines on Lilium longiflorum pollen germination under high temperature stress |
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YANG Yanhong, PEI Baozhi, BU Xinyu, SUN Minghan, GUO Hongsha, ZHUANG Jianhao, JIANG Qianqian
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School of Advanced Agriculture Sciences，Weifang University，Weifang，Shandong 261000，China
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| Abstract: |
| 【 Objective】The study aimed to investigate the regulatory effects of exogenous polyamines（spermine，spermidine） on Lilium longiflorum Thunb. pollen germination under high temperature stress.【Method】Fresh L.longiflorum pollen was used as the material.After optimizing the in vitro culture conditions，the pollen was subjected to temperature treatments of 25，30，35，and 40 ℃. By measuring the germination rate and pollen tube length of lily pollen under different temperature treatments，35 ℃ was selected as the high temperature stress condition for subsequent experiments. Pollen was then cultured under 25 ℃（control） and 35 ℃（high temperature stress） conditions，with the addition of Spd or Spm at concentrations of 0（control），0.025，0.05，and 0.1 mmol/L. The pollen germination rate，pollen tube length，distortion rate，viability，reactive oxygen species distribution，and callose distribution were assessed under these different treatments.【Result】① The optimal liquid medium for in vitro culture of L.longiflorum pollen was determined to contain：10% sucrose，0.06% boric acid，0.02% calcium chloride，0.02% magnesium sulfate，and 0.02% potassium dihydrogen phosphate. ② Compared with the control（25 ℃），high temperature stress significantly inhibited pollen germination and pollen tube growth，leading to abnormal morphological changes including tube rupture，bending，and apical swelling. ③ Under 35 ℃ high temperature stress，compared with the control，the addition of 0. 05 mmol/L Spd significantly increased the pollen germination rate and pollen tube length by 24.78% and 16.36%，respectively. The addition of 0.025 mmol/L Spm increased the germination rate by 7.22%，but inhibited pollen tube elongation. The addi?tion of 0. 05 mmol/L Spd or 0.025 mmol/L Spm reduced the pollen tube distortion rate by 21.65% and 26.69%，respectively. These treatments effectively alleviated the abnormal reactive oxygen species distribution and disordered callose deposition caused by high temperature，thereby improving pollen tube viability.【Conclusion】High temperature stress significantly inhibited lily pollen germination and pollen tube growth，leading to pollen tube distortion. The addition of 0.05 mmol/L spermidine（Spd）effectively restored pollen germination and normal pollen tube elongation under high temperature，whereas spermine（Spm） only promoted germina?tion. Exogenous polyamines significantly alleviated high temperature damage mainly by restoring ROS signaling and callose distribution in pollen tubes，thereby improving pollen tube viability. |
| Key words: Lilium longiflorum. high temperature stress pollen germination pollen tube elongation pollen tube morphology ROS callose deposition |
