| 摘要: |
| 【目的】探究外源糖调控彩色马蹄莲佛焰苞花青素苷合成和呈色的分子机制，为切花采后保鲜技术的开发提供理论依据。【方法】以玫红色彩色马蹄莲切花为试材，研究333，499，666 mmol/L的葡萄糖、甘露糖、甘露醇、GHG(盐酸氨基葡萄糖和葡萄糖混合溶液)处理对切花色彩表型、花青素苷含量及其合成相关基因表达的影响。【结果】葡萄糖、甘露糖和甘露醇处理使彩色马蹄莲佛焰苞明度下降，红度和彩度增加，并可促进花青素苷的积累；GHG处理显著降低佛焰苞红度和彩度，使花青素苷积累下降。499 mmol/L甘露醇处理显著促进了花青素苷合成相关基因ZhMYB、ZhCHS、ZhCHI、ZhF3H、ZhF3′H、ZhDFR和ZhANS的表达，葡萄糖处理显著促进了除ZhANS外6个基因的表达；GHG处理下ZhMYB、ZhCHS和ZhCHI的表达量低于葡萄糖单独处理。【结论】适量的外源葡萄糖、甘露糖和甘露醇能有效促进彩色马蹄莲佛焰苞中花青素苷的积累并改善其呈色，且499 mmol/L葡萄糖、甘露醇可以显著促进花青素苷合成相关基因的表达，其中葡萄糖通过依赖HXK途径调控ZhMYB、ZhCHS和ZhCHI的表达以影响佛焰苞中花青素苷的合成。 |
| 关键词: 彩色马蹄莲 外源糖 色彩表型 花青素苷 糖信号 基因表达 |
| DOI：10.13207/j.cnki.jnwafu.2025.11.016 |
| 分类号: |
| 基金项目:国家自然科学基金项目(31760587)；云南省大学生创新创业训练计划项目(S202210674027) |
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| Effects of exogenous sugar on color phenotype and anthocyanin synthesis in the spathe of Zantedeschia hybrida |
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LÜ Chengcong, ZHANG Le, ZHAO Meng, ZHANG Zijun, HUANG Shasha, JIN Xuehua
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School of Architecture and Urban Planning,Kunming University of Science and Technology,Kunming，Yunnan 650000,China
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| Abstract: |
| 【Objective】This study aimed to reveal the molecular mechanism of anthocyanin synthesis and coloration in the spathe of Zantedeschia hybrida regulated by exogenous sugars,which can provide theoretical basis for the development of postharvest preservation technology of cut flowers.【Method】The cut flowers of pink Z. hybrida were used as materials to study the effects of glucose,mannose,mannitol,and GHG (mix of glucosamine hydrochloride and glucose) on the color phenotype,anthocyanin content,and expression of the genes related to their synthesis at 333 mmol/L,499 mmol/L,and 666 mmol/L,respectively.【Result】Treatment of glucose,mannose,and mannitol reduced spathe brightness,increased redness and chroma,and promoted anthocyanin accumulation.GHG treatment reduced spathe redness and chroma,and decreased anthocyanin accumulation.499 mmol/L mannitol promoted the expression of anthocyanin synthesis-related genes,including ZhMYB,ZhCHS,ZhCHI,ZhF3H,ZhF3′H,ZhDFR,and ZhANS,while glucose promoted the expression of six genes except ZhANS.Expression of ZhMYB,ZhCHS,and ZhCHI under GHG treatment was lower than glucose alone.【Conclusion】Appropriate amount of exogenous glucose,mannose,and mannitol can effectively promote anthocyanin accumulation in the spathe of Z.hybrida and improve color appearance.At 499 mmol/L,glucose and mannitol significantly promote the expression of anthocyanin synthesis related genes,in which glucose may regulate the expression of ZhMYB,ZhCHS,and ZhCHI through the HXK pathway. |
| Key words: Zantedeschia hybrida exogenous sugar color phenotype anthocyanins sugar signal gene expression |
