| 摘要: |
| 【目的】改良国家优质米NJ43的稻瘟病抗性。【方法】以YF47×NJ43杂交的水稻 RIL F5-6群体作为研究对象，采用喷雾法人工接种和自然诱发二者相结合的方法对其进行稻瘟病抗性鉴定，通过 PCR 扩增技术检测稻瘟病抗性基因的组成，并通过qRT-PCR 技术检测基因表达水平，分析抗稻瘟病（R）基因pib、pid2、<Pik-h、pikm、pi5、pi9在水稻材料中的分布、基因组合、基因表达量及与稻瘟病抗性的关系。【结果】在RIL群体中达到中感的材料最多，占RIL群体的65.11%，达到抗病、中抗和感病的材料分别占RIL群体的13.95%，9.30%和11.63%，没有达到高感和高抗的材料。各抗性基因Pik-h、pib、 pid2、pikm、pi5和pi9在供试材料中出现的频率分别为100.00%，90.70%，74.42%，60.47%，69.77%和46.51%，且这些R基因聚合成了14种不同类型的基因型，不同基因型所含抗性基因数量从1~6个不等，且随着抗性基因数量的增加抗性不是递增，说明不同抗性基因发挥的作用及基因聚合效应不同。通过对不同基因型携带的基因与RIL的稻瘟病抗性进行多元回归分析发现，抗性基因pid2和Pik-h对稻瘟病抗性有正向的显著贡献，而pikm基因对稻瘟病抗性有负向的显著贡献，pi5基因对稻瘟病抗性有负向的极显著贡献。对不同基因型的平均抗性及聚合效应分析发现，基因型7、8和14所聚合的基因Pik-h、pid2和pib之间存在显著的基因重叠效应、互补效应和正向聚合效应；基因型4（pib+Pik-h+pikm+pi9）、基因型8（pid2+Pik-h）和基因型14（pib+pid2+Pik-h）的抗性显著高于其他基因型，且存在显著的正向互作效应。对不同基因的表达量分析发现，pid2、pikm和pi9基因的表达量在接种后7 d达到最高，Pik-h和pib基因的表达量在接种后48 h达到最高，且在RIL群体中的表达量均显著高于亲本（YF47），在抗性材料中的表达较感病材料显著上调，说明其在RIL群体中能有效高表达。【结论】抗性基因pid2和Pik-h及基因型pid2+Pik-h、pib+pid2+Pik-h和 pib+Pik-h+pikm+pi9可作为宁夏水稻抗稻瘟病育种中的主要抗性基因及基因聚合模式。 |
| 关键词: 粳稻 稻瘟病 基因聚合 抗性鉴定 |
| DOI：DOI:10.13207/j.cnki.jnwafu.2025.06.007 |
| 分类号: |
| 基金项目:宁夏自然科学基金重点项目（2022AAC02008） |
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| Polymerization effect and expression analysis of blast resistance genes in RIL population from Japonica rice |
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SU Sirong1, GUAN Ruijie1, ZHANG Yuzhi1, TIAN Lei1,2, ZHANG Yinxia1,2
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1.School of Agriculture,Ningxia University,Yinchuan,Ningxia 750021,China;2.Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan,Ningxia 750021,China
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| Abstract: |
| 【Objective】This research aimed to improve the rice blast resistance of the national high quality rice cultivar NJ43.【Method】Rice RIL F5-6 population of the YF47×NJ43 cross was used in this study,and the blast resistance was identified by a combined method of spray artificial inoculation and natural induction.The composition of blast resistance genes was detected by PCR amplification,and the gene expression levels were detected by qRT-PCR.The distribution,gene combinations,gene expression level and relationship with rice blast resistance (R) of genes pib,pid2,Pik-h,pikm,pi5 and pi9 in rice materials were analyzed.【Result】In RIL population,the materials that achieved medium sensitivity were the most,accounting for 65.11%,and the materials of resistance,medium resistance and susceptibility accounted for 13.95%,9.30% and 11.63% of the RIL population,respectively.No material achieved high sensitivity or high susceptibility.The frequencies of resistance genes Pik-h,pib,pid2, pikm,pi5 and pi9 in the test materials were 100.00%,90.70%,74.42%,60.47%,69.77% and 46.51%,respectively.These R genes formed 14 different genotypes after aggregation,and the R gene numbers of different genotypes ranged from 1-6.The resistance of RIL did not increase with the increase of the number of R genes,which indicated that each R genes played different roles and the effect of gene polymerization was different. Through multiple regression analysis of genes carried by different genotypes and the resistance of RIL,it was found that pid2 and Pik-h genes had significant positive correlation with blast resistance,pikm gene had significant negative correlation with blast resistance,and pi5 gene had highly significant negative effect on blast resistance.Analysis on the average resistance and polymerization effect of different genotypes showed that there were significant overlapping,complementary and positive polymerization effects among Pik-h,pid2 and pib polymerized in genotypes 7,8 and 14.The resistance of genotype 4 (pib+Pik-h+pikm+pi9),genotype 8 (pid2+Pik-h) and genotype 14 (pib+pid2+Pik-h) was significantly higher than that of other genotypes and there were significant positive interaction effects.Improving the resistance of different R gene polymers required not only high complementary effects among genes but also effective expression.So the expression of different genes was analyzed and it was found that the expression of pid2,pikm and pi9 genes reached the highest level at 7 d,and the expression of Pik-h and pib genes reached the highest level at 48 h.All of them were significantly higher than that of the parental material (YF47) in the RIL population,and the expression of these genes was significantly up-regulated in the resistant material compared with that of the disease susceptible material,which indicated that they can be effectively expressed at high levels in the RIL.【Conclusion】R genes pid2 and Pik-h and the genotypes pid2+Pik-h,pib+pid2+Pik-h and pib+Pik-h+pikm+pi9 can be used as the main resistance genes and gene polymerization patterns in the breeding of Ningxia rice for resistance to rice blast. |
| Key words: Japonica rice rice blast gene polymerization resistance identification |
