| 摘要: |
| 【目的】优化高压脉冲电场辅助（PEF）法从人参药材中提取多种水溶物的工艺条件。【方法】以电场强度、脉冲数及料（g）液（mL）比为因变量，以人参皂苷、人参多糖、AF及AFG提取率为指标，在单因素试验基础上，设计3因素3水平正交试验，用PEF法提取人参中水溶性物质并对工艺参数进行优化,最后与用煎煮法和超声法对各提取物的提取率进行比较。【结果】用PEF法提取人参多种水溶物的最佳工艺条件为：电场强度25 kV/cm，脉冲数10，料（g）液（mL）比1∶30。在此条件下，人参水提物中的皂苷、多糖、AF和AFG提取率分别为0.98%，7.20%，0.19%，0.11%；且各成分提取率均高于煎煮法及超声法。【结论】优化了人参中水溶物的提取工艺。 |
| 关键词: 高压脉冲电场法 人参皂苷 人参多糖 AF AFG |
| DOI： |
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| 基金项目:吉林省科技发展计划项目（20140204063YY）；长科技合（2013177）号计划项目（13NK06） |
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| Optimization of high voltage pulse electric field assisted extraction of ginseng aqueous extracts |
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FAN Chao,XU Lingzhi,SUN Changbo,et al
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| Abstract: |
| 【Objective】This study aimed to improve the extraction of ginseng aqueous extracts assisted by high voltage pulsed electric field (PEF).【Method】The effects of electric field intensity,number of pulses and material water ratio on extraction yields of ginsenoside,ginseng polysaccharide,AF and AFG were tested based on single factor experiments.Then orthogonal experiments of the three factors at three levels were conducted to optimize the extraction process and the yields were compared with other methods.【Result】The optimal conditions of PEF process to extract ginseng aqueous extracts were electric field intensity 25 kV/cm,number of pulses 10 and material water ratio 1∶30.The yields of ginsenoside,polysaccharide,AF and AFG were 0.98%,7.20%,0.19%,0.11%,respectively.All the yields were higher than other methods. 【Conclusion】The extraction process had been optimized and the PEF technology can be used for extraction of ginseng aqueous extracts. |
| Key words: high voltage pulsed electric field ginseng ginsenoside ginseng polysaccharide AF AFG |
