| 摘要: |
| 【目的】探究魔芋葡甘聚糖(Konjac glucomannan，KGM)对饮酒大鼠血清硒及脂质过氧化的影响。【方法】将45只供试雄性SD大鼠随机分成正常对照组、酒精肝模型组、质量分数5%和10%KGM预防组及质量分数10%谷胱甘肽(GSH)阳性对照组，每组9只，进行相应处理，12周后，心脏无菌穿刺采血，制备血清，并处死大鼠，取其肝组织，测定血清中的硒含量及肝组织谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)、GSH、H2O2的含量。【结果】与其他各组相比，酒精肝模型组大鼠血清硒水平极显著降低(P<0.01)；KGM预防组大鼠血清硒水平与GSH阳性对照及正常对照组均无显著性差异(P>0.05)。大鼠肝脏GSH-Px活性与血清硒水平呈极显著正相关(r=0.542，P=0.001)。与其他各组相比，酒精肝模型组SOD活性极显著降低(P<0.01)，GSH-Px活性及GSH含量显著或极显著降低(除5%KGM预防组GSH-Px活性外)，MDA、H2O2含量均极显著升高(P<0.01)；2个KGM预防组与正常对照组和阳性对照组各指标水平较为接近。【结论】KGM能有效发挥其抗氧化应激作用，从而预防乙醇导致的大鼠肝脏脂肪变性。 |
| 关键词: 大鼠 酒精型脂肪肝 葡甘聚糖 抗氧化 |
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| 基金项目:陕西省自然科学基金项目(2010JM4052)；陕西省教育厅科技专项(12JK0848) |
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| Antioxidant effect of KGM on serum selenium and lipid peroxidation in rats with chronic alcohol abuse |
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| Abstract: |
| 【Objective】This study aimed to analyze the antioxidant effect of KGM on serum selenium and lipid peroxidation in rats with chronic alcohol abuse.【Method】Male SD rats were divided into 5 groups:normal control group,drinking alcohol group,5%KGM preventive group,10%KGM preventive group,and 10% GSH positive control group.After the 12 weeks of experiment,the rat serum selenium levels were measured by atomic fluorescence spectrometry.Hepatic GSH-Px and SOD activity were evaluated and GSH,MDA and H2O2 levels were determined using an assay kit.【Result】After 12 weeks,compared with the other groups,serum selenium levels of rats in drinking alcohol group were significantly reduced(P<0.01).There was no significant difference(P>0.05)between rats in preventive group and rats in both GSH positive control group and normal control group.The experimental results showed that the liver GSH-Px activity and serum selenium were positively correlated(r=0.542,P=0.001).Compared with the other groups,SOD activity of rats in drinking alcohol group decreased significantly(P<0.01),GSH-Px activity and GSH content significantly decreased (except GSH-Px of rats in 5% KGM prevention group),and MDA and H2O2 content increased significantly (P<0.01).Each index level of rats in prevention group was relatively close to that of rats in both normal control group and positive control group.【Conclusion】The KGM has antioxidant effect on serum selenium and lipid peroxidation in rats with chronic alcohol abuse. |
| Key words: rat alcoholic fatty liver KGM antioxidant |
