| 摘要: |
| 【目的】 研究甜玉米株高的遗传模式和QTL定位，为玉米高产、耐密和抗倒伏育种提供理论依据。【方法】 以株高有显著差异的甜玉米自交系T14和T4为亲本配制杂交组合，采用主基因+多基因混合遗传模型和P1、P2、F1、B1、B2和F2 6个世代联合分析的方法,对甜玉米株高性状进行遗传分析；以330个F2单株为作图群体，采用复合区间作图法和群体分离分析法（BSA法），在F2和F2:3家系中检测株高QTL。【结果】 玉米株高受2对加性-显性-上位性主基因控制，在各个分离世代都以主基因遗传为主；在F2群体中，检测到的3个QTL位于第1染色体，2个QTL位于第5染色体上，对表型变异的贡献率为7.8%～28.8%；在F2:3家系中，检测到的4个QTL位于第1染色体，4个QTL位于第5染色体上，对表型变异的贡献率为4.8%～27.4%。【结论】 在F2和F2:3家系中检测到的株高QTL都集中在第1和第5染色体上，形成了2个明显的株高QTL簇，这一结果与2对主基因+多基因的遗传模型相吻合。 |
| 关键词: 甜玉米 株高 遗传模型 QTL |
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| 基金项目:广州市科技支撑计划项目（2008Z1-E541）；广东高校优秀青年创新人才培育项目（K1101201）；仲恺农业工程学院引进优秀人才科研启动基金项目（G2360305）；仲恺农业工程学院自然科学基金项目（G3091202） |
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| Genetic analysis and QTL mapping for plant height in corn |
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JIANG Feng1, LIU Pengfei1, ZENG Muheng2
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1.College of Agronomy ,Zhongkai University of Agriculture and Engineering;2.College of Life Sciences, Zhongkai University of Agriculture and Engineering
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| Abstract: |
| 【Objective】The aim was to study the genetic mode and QTL mapping of corn plant height for providing theoretical basis to high yield,density and lodging resistance breeding.【Method】Cross combination was designed by selecting two sweet corn inbred (T14 and T4) different significantly in plant height.Genetic segregation analysis for plant height was evaluated using major gene polygene mixed inheritance models and joint analysis of P1,P2,F1,B1,B2 and F2 populations was done.Using 330 F2 individuals as genetic mapping population,we carried out QTL screening with the methods of bulked segregant analysis (BSA) and multiple interval mapping (CIM).【Result】Plant height of sweet corn inbred T14 was controlled by two major genes with additive-dominance-epistatic effects,and the inheritance of the major gene was dominant.3 QTL on chromosome 1 and 2 QTL on chromosome 5 were detected by F2 population,accounting for 7.8%-28.8% of phenotypic variance.Moreover,4 QTL on chromosome 1 and 4 QTL on chromosome 5 were screened by F2:3 population,controlling 4.8%-27.4% of variance.【Conclusion】The QTL mapping result revealed that QTL clusters with high contribution rates were screened simultaneously on chromosome 1 and 5.This result is consistent with the genetic model of two major genes in T14. |
| Key words: sweat corn plant height genetic model QTL |
