| 摘要: |
| [目的]为紫外诱变技术在环境工程中的应用提供参考。[方法] 由1株絮凝剂产生菌XL-18出发,经过紫外诱变得到絮凝活性高的正突变菌株YB-1,用红外光谱、薄层色谱分析纯化后YB-1产微生物絮凝剂的成分,苯酚-硫酸法分析样品中糖含量,双缩脲法分析样品中蛋白质含量,并用YB-1产微生物絮凝剂处理水性油墨废水,研究了不同因素(pH、投加量、助凝剂)对絮凝效果的影响。[结果] 菌株YB-1的絮凝率为95。0%；YB-1产微生物絮凝剂含有羟基、羧基等官能团,总糖含量为6。630 g/L,不含蛋白质,其是由葡萄糖和甘露糖为主要单体组成的多糖；添加0。5 mol/L CaCl2溶液作助凝剂、废水pH 8。0、YB-1发酵液投加量为60 ml/L时,水性油墨废水的COD、浊度、色度去除率分别为88。25%,94。55%和95。27%,并得到了该微生物絮凝剂的动力学方程。[结论] 紫外诱变技术可以明显提高微生物絮凝剂的絮凝活性,是获得高效微生物絮凝剂产生菌的有效途径。 |
| 关键词: 紫外诱变 微生物絮凝剂 水性油墨废水 动力学方程 |
| DOI： |
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| 基金项目:2006年第一批中央环境保护专项基金（财建［2006］859号） |
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| Mutation breeding of bioflocculant-producing strain with high-efficiency by ultraviolet and its application |
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| Abstract: |
| 【Objective】 The study was done to provide reference for the application of UV induced mutation technology in environmental engineering.【Method】 A high efficient bioflocculant strain YB-1 has been obtained by UV induced mutation from an inefficient strain named XL-18.The bioflocculant element was obtained with TLC and analyzed with IR.The sugar content and protein content were analyzed with Phenol-sulfate quantitative analysis and Biuret law.The wastewater of water-based ink was treated by YB-1 to investigate the effects of pH,dose and coagulantaids on flocculation.【Result】 The flocculant efficiency of YB-1 was 95%.The IR spectrum showed that flocculant YB-1 contains hydroxyl,carboxyl etc.functional groups,total sugar content 6.630 g/L,no proteins.The flocculant consisted of mannose and glucose,as well as other monomers.The removal rate of COD,turbidity and chroma were 88.25%,94.25% and 95.27 respectively,when pH was 8.0;inoculum was 60 ml/L and 0.5 mol/L CaCl2 as coagulant aid.In addition,the dynamics equation of deposition was deduced.【Conclusion】 UV induced mutation can improve flocculation activity significantly.It is an effective way to breed bioflocculant-producing strain with high efficiency. |
| Key words: UV mutagenesis microbial flocculant wastewater of wwater-based ink dynamics |
