| 摘要: |
| [目的]为了更好地了解NDRG1的功能,通过双向电泳技术寻找与NDRG1相互作用的蛋白.[方法]先准备高表达肿瘤细胞系HHCC,然后纯化融合蛋白GST-NDRG1,最后孵育HHCC与融合蛋白,并通过双向电泳检测与NDRG1相互作用的蛋白.[结果]经SDS-PAGE和双向电泳后,在pH 7.0、Mr=43 ku处出现了新增点.[结论]在肿瘤细胞HHCC中存在与NDRG1相互作用的蛋白. |
| 关键词: GST-NDRG1 双向凝胶电泳 人肝癌细胞系 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30370315，30171044） |
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| Two-dimensional Gel Electrophoresis for the interact protein with NDRG1 |
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| Abstract: |
| 【Objective】 The study was to better realize the function of NDRG1 and find the interactive protein through two-dimensional gel electrophoresis technology.【Method】 First,the cell of HHCC was separated.Second,the fusion protein GST-NDRG1 was purified.Third,the fusion protein with HHCC was incubated and the interactive protein with NDRG1 was observed through two-dimensional gel electrophoresis technology.【Result】 There was a new dot emergence at pH 7.0 and Mr 43 ku after two-dimensional gel electrophoresis.【Conclusion】 The interactive protein with NDRG1 was found in HHCC. |
| Key words: GST-NDRG1 two-dimensional gel electrophoresis HHCC |
