| 摘要: |
| [目的]优化百部总生物碱的提取工艺,为其在农业、医药、食品等方面的开发提供理论参数.[方法]在单因素试验的基础上,选取百部总生物碱提取温度、提取时间和乙醇质量分数3个因素进行Box-Benhnken中心组合设计,利用响应面分析法对其提取工艺参数进行优化.[结果]在提取温度57.76℃,提取时间13.18 h,乙醇质量分数75.21%时,百部总生物碱的理论含量最高,可达9.76 mg/g.根据实际试验情况,将其修正为提取温度57.8℃,提取时间13.2 h,乙醇质量分数75.2%,经验证此条件下百部总生物碱含量为9.63 mg/g,与理论值较为接近.[结论]修正后的百部总生物碱提取工艺优化参数准确可靠,有一定的实用价值. |
| 关键词: 百部 总生物碱 响应面分析法 提取工艺 |
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| 基金项目:河南省重大科技攻关项目（0322010900） |
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| Optimization of extraction technique of total alkaloid from Radix Stemona via Response Surface Methodology |
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| Abstract: |
| 【Objective】 The extraction technique of total alkaloid from Radix Stemona was optimized to provide theoretical parameters for its exploiture in agriculture,medicament,foodstuff and so on.【Method】 On the basis of single-factor experiments,the effects of different temperature,time and ethanol weight percent on the extraction yield of total alkaloid from Radix Stemona were investigated with a three-factor Box-Behnken center-united design. The extraction parameters were optimized with Response Surface Methodology.【Result】 The results indicated that the conditions of 57.76 ℃,75.21% ethanol and heating and refluxing for 13.18 h were optimum, and the yield of total alkaloid could be up to 9.76 mg/g.According to conditions of actual experiment,the optimum extraction parameters were modified at 57.8 ℃ for 13.2 h with ethanol weight percent 75.2%.The validated experiment showed the yield of total alkaloid was 9.63 mg/g under those conditions which was in consistency with the theoretic value.【Conclusion】 The modified extraction parameters were credible and had some practical value |
| Key words: Radix Stemona total alkaloid response surface methodology(RSM) extraction technique |
