| 摘要: |
| [目的]研究小麦抗条锈菌的分子机制,在受条锈菌侵染的非亲和组合小麦叶片中克隆一个新的β-1,3-葡聚糖酶基因全长cDNA.[方法]采用RT-PCR、cDNA末端快速扩增技术(RACE),在感染小麦条锈菌的小麦(水源11)叶片中进行β-1,3-葡聚糖酶基因全长cDNA的扩增.[结果]得到了一个1 338 bp β-1,3-葡聚糖酶基因全长cDNA,Genbank上注册号为DQ090946,测序结果和序列生物信息学分析结果表明,其与β-1,3-葡聚糖酶基因gi3757681和gi6782375的同源性分别为94.3%和92.1%.该全长cDNA具有完整的编码框,编码334个氨基酸,Genbank上注册号为AAY96422,与β-1,3-葡聚糖酶CAA77085和AAA32958的同源性分别为95.8%和86.7%.[结论]初步推断此全长cDNA是小麦中编码β-1,3-葡聚糖酶的一个新基因. |
| 关键词: 小麦 条锈菌 β-1,3-葡聚糖酶基因 分子克隆 全长cDNA RACE |
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| 基金项目:国家“863”项目“小麦抗病性状功能基因组研究”（2006AA10A104）；国家“973”前期专项（2006CB708208）；教育部重大科技培育项目（2004 295）；教育部长江学者和创新团队发展计划项目（IRT0558）；国家自然科学基金项目（30671350）；高等学校学科创新引智计划项目（B07049） |
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| A full-length cDNA cloning of a wheat β-1,3-glucanase gene using rapid amplification of cDNA ends |
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| Abstract: |
| 【Objective】 The paper studied the molecular mechanisms of wheat resistance to Puccinia striiformis.A full length cDNA of a β-1,3-glucanase gene was cloned in wheat leaves which were infected by Puccinia striiformis in incompatible interaction.【Method】 RT-PCR and Rapid Amplification of cDNA Ends(RACE) technique was used to obtain a full length cDNA of a β-1,3-glucanase gene in wheat leaves which were infected by Puccinia striiformis.【Result】 The full length cDNA has 1 338 bp nucleotides,the GenBank accession number is DQ090946,the sequence analysis result shows that it has 94.3%,92.1% identity with gi3757681,gi6782375.it has the complete open reading frame and is classified as 334 amino acids with codes,the GenBank accession number is AAY96422,it has 95.8%,86.7% identity with CAA77085,AAM75342.【Conclusion】 The full length cDNA is supposed to be a new gene of β-1,3-glucanase Gene in Wheat leaves. |
| Key words: wheat Puccinia striiformis β-1,3-glucanase gene molecuar cloning full length cDNA RACE |
