| 摘要: |
| 采用大鼠心肌细胞条件培养基对兔胚胎干细胞(Embryonic stem cells,ESC)进行分离、传代培养,研究大鼠心肌细胞条件培养基对兔ESC分离培养效果的影响。结果显示,用SD大鼠心肌细胞条件培养基培养的兔ESC集落呈岛屿状生长,碱性磷酸酶染色呈强阳性,体外分化可形成类胚体状结构,贴壁的类胚体周边会出现许多分化的上皮样细胞或单个散在的细胞;常规冻存后再传代的兔ESC集落具有较为一致的生长特征,并呈现胚胎干细胞集落所特有的岛屿状生长形态;传至第5代的兔ESC集落核型正常率>75％。表明SD大鼠心肌细胞条件培养基可用于兔胚胎干细胞分离培养。 |
| 关键词: 兔 细胞培养 大鼠心肌细胞条件培养基 类胚胎干细胞 |
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| 基金项目:河南省杰出人才创新基金项目（0421002100） |
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| Effect on isolation and culture of rabbit embryonic stem cells with rat cardiomyocyte conditioned medium |
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| Abstract: |
| Effect on isolation and culture of rabbit embryonic stem (ES) cells with rat cardiomyocyte conditioned medium was studied.Results showed that isolated rabbit ES-like cells clusters cultured by SD rat cardiomyocyte conditioned medium were island-like;alkaline phosphatase staining of rabbit ES-like cell was strongly positive;rabbit ES-like cells could become embryonic bodies-like structures in vitro,and embryonic bodies which stuck to plates could be differentiated into epithelial-like cells or scattered single cell;rabbit ES cells cluster were identical island-like image and grew coincidently by culture after routin freeze;karyotype analysis of the 5th passage rabbit ES cells was researched,and the karyotype normal rate was over 75%.The essay showed SD rat cardiomyocyte conditioned medium can be used to isolation and culture of rabbit ES-like cell. |
| Key words: rabbit,cells culture,rat cardiomyocytes conditioned medium,embryonic stem-like cells |
