| 摘要: |
| 对东方百合杂交系(Oriental hybrids)中西西(Sissi)品种的叶片、叶柄进行了离体培养研究。结果表明，诱导西西叶片、叶柄分化出小鳞茎的培养基为S4(MS +6-BA 2.0 mg/L +NAA 0.2 mg/L)，诱导率分别为22.2％，11.1％。S4培养基诱导的小鳞茎粗壮，再分化能力强，诱导率相对较高，宜采用MS +6-BA 2.0 mg/L +NAA 0.2 mg/L培养基作为继代培养基；生根培养基为1/2MS +IBA 0.3 mg／L。 |
| 关键词: 东方百合 组织培养 叶片 叶柄 小鳞茎 |
| DOI： |
| 分类号:S682.29 |
| 基金项目:上海市农工商现代农业园区横向合作项目(141107) |
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| Tissue culture of oriental lily hybrids |
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ZHANG Yan-long,XU Yan,WANG Jie-chun,YANG Zhi-juan,ZHANG Yan-long,XU Yan,WANG Jie-chun,YANG Zhi-juan,ZHANG Yan-long,XU Yan,WANG Jie-chun,YANG Zhi-juan
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| Abstract: |
| The leaf,petiole of the oriental lily hybrids,Sissi was in vitro cultured.The optimal medium for the leaf,petiole of Sissi was S4 medium (MS+6-BA 2.0 mg/L+NAA 0.2 mg/L),and the inducing rate of bublets was 22.2%,11.1% respectively.The bulblets differentiated on the S4 (MS+6-BA 2.0 mg/L+NAA 0.2 mg/L) medium was better,and the inducing rate was higher.The subculture medium was the S4 (MS+6-BA 2.0 mg/L+NAA 0.2 mg/L) medium.The rooting medium was 1/2 MS+IBA 0.3 mg/L. |
| Key words: oriental lily hybrids tissue culture leaf petiole bublet |
