| 摘要: |
| 采用混合培养法研究接菌、加氮对未灭菌和灭菌鲜苹果渣发酵生产饲料蛋白的影响。结果表明,①接菌后鲜苹果渣的纯蛋白含量显著提高,其中未灭菌鲜苹果渣接入黑曲霉UA8发酵后,纯蛋白含量较未接菌的增加了137.8%。②加氮处理后鲜苹果渣纯蛋白含量高于不加氮处理,其中未灭菌鲜苹果渣接入黑曲霉UA8后加氮发酵产物纯蛋白含量较不加氮对照增加了35.2%。③未灭菌鲜苹果渣发酵后纯蛋白含量高于灭菌处理,其中单菌有氮发酵处理中,接入黑曲霉UA8时纯蛋白含量分别为105.6和75.0 g/kg,直接发酵较灭菌发酵纯蛋白增加了40.8%。④鲜苹果渣发酵后发酵产物回收率与其纯蛋白含量呈负相关,未灭菌鲜苹果渣发酵有氮和无氮处理中,接入黑曲霉UA8后,发酵产物纯蛋白含量分别为105.6和78.1 g/kg,而其回收率分别为62.2%和81.0%。⑤不同pH时鲜苹果渣发酵产物纯蛋白含量呈pH 4>pH 6的趋势,未灭菌鲜苹果渣发酵中果渣酸度为pH 4和pH 6时,酵母菌发酵产物纯蛋白含量分别为101.2和75.9 g/kg,果渣自然酸化使发酵产物纯蛋白增加了33.3%。 |
| 关键词: 苹果渣 饲料蛋白 发酵饲料 单细胞蛋白 固态发酵 酵母菌 黑曲霉 |
| DOI： |
| 分类号:S816.4 |
| 基金项目:国家科技部西部专项基金项目(2001BA901A19) |
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| The fermentation of fresh pomace producing feeding-protein |
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CHANG Xian-bo~1,XUE Quan-hong~1,LAI Hang-xian~1,YUE Tian-li~2,XIN Jian-kang~1,CHANG Xian-bo~1,XUE Quan-hong~1,LAI Hang-xian~1,YUE Tian-li~2,XIN Jian-kang~1,CHANG Xian-bo~1,XUE Quan-hong~1,LAI Hang-xian~1,YUE Tian-li~2,XIN Jian-kang~1
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| Abstract: |
| The effect was studied by mixed culture on adding some inorganic nitrogen and inoculation about feeding protein content of fresh pomace with sterilization and without sterilition.The result showed:① The protein content of fresh pomace inoculated was raised remarkablely,in which the without sterilization and incoculation with Aspergillus UA8 increased by 137.8% compared with no incoculation in the fermentation.② The protein content of fresh pomace after adding inorganic nitrogen was higher than that without inorganic nitrogen,the former that was not sterilizated and inoculated with Aspergrillus UA8 was 35.2% higher than the latter.③ The protein content decreased after the fresh pomace was sterilized.The protein content of fresh pomace inoculated with Aspergillus UA8 and added inorganic nitrogen was 105.6 and 75.0 g/kg,in which the sterilizated was 40.8% higher than that without sterilization.④ The rate of recovery and the protein content were contrary.During the fermentation of fresh pomace without sterilization,the protein content of fresh pomace that inoculated with Aspergillus UA8 was 105.6 and 78.1 g/kg in the circumstances of adding inorganic nitrogen and no nitrogen,and its rate of recovery was 62.2% and 81.0% respectively.⑤ The protein content presented the tendency of pH4>pH6 in the fermentation.The protein content of fresh pomace without sterilization and inoculation with yeast was 101.2 and 75.9 g/kg at pH4 and pH6,so the protein content in the product exceeded 33.3% by natural acidification of pomace. |
| Key words: pomace feeding-protein feed fermentation single cell protein solid fermentation yeast Aspergillus niger |
