| 摘要: |
| 比较了添加LIF，IGF－1和LIF＋IGF对牛胚胎干细胞（ES）分离的影响。结果表明：（1）添加10 ng/mL LIF能提高胚胎贴壁率，促进内细胞团（ICM）和ES细胞的增殖并抑制其分化；（2）添加10 ng/mL IGF-1既能提高胚胎贴壁率，又能使ICM明显增殖的时间和传代后集落出现的时间提前；（3）添加10 ng/mL LIF +10 ng/mL IGF-1，可以同时发挥两种生长因子的作用效果，更有利于牛胚胎干细胞的分离。 |
| 关键词: LIF IGF-1 胚胎干细胞 牛胚胎 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(39670359)；国家“九五”攻关项目(96-C01-03) |
|
| Influences of LIF and IGF-1 on the isolation of bovine ES cells |
|
|
| Abstract: |
| In this paper,we compared the influences of LIF,IGF-1 and LIF+IGF-1 on the isolation of bovine ES cells.The results showed that:① Supplementing with 10 ng/mL LIF could enhance the ration of embryo attachment,promote the proliferation of ICM and ES cells and inhibit the differentiation of ICM and ES cells;② Supplementing with 10 ng/mL IGF-1 could move up the time for the sufficient proliferation of ICM and ES cells.③ Supplementing with 10 ng/mL LIF+10 ng/mL IGF-1,both the effects of LIF and IGF-1 were brought into play. |
| Key words: ILF IGF ES cells bovine |
