| 摘要: |
| 通过反转录PCR(RT-PCR)测定了肝窦内皮细胞和枯否细胞中bcl-2和GAPDH rmRNA的半衰期。结果表明，在窦内皮细胞中bcl-2和GAPDH mRNA的半衰期分别为6.13和大于20 h,在枯否细胞中bcl-2和GAPDH rmRNA的半衰期分别为5.86和13.74 h。 |
| 关键词: RT-PCR 窦内皮细胞 枯否细胞 mRAN半衰期 |
| DOI： |
| 分类号:S663.1 |
| 基金项目:德国DFG基金资助项目 |
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| Half life determination of bcl-2 and GAPDH mRNA in sinusoidal endothelial cells and Kupffer cells of rat liver |
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WANG Yue jin 1,ZHANG Jian xia 1,ZHOU Peng 2,ZHENG Xue qin 2,WANG Yue jin 1,ZHANG Jian xia 1,ZHOU Peng 2,ZHENG Xue qin 2,WANG Yue jin 1,ZHANG Jian xia 1,ZHOU Peng 2,ZHENG Xue qin 2
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| Abstract: |
| In the study,half lives of bcl-2 and GAPDH mRNA were determined in isolated sinusoidal endothelial cells (SEC) and Kupffer cells (KC) of rat livers during Thioacetamide-induced Fibrosis by RT-PCR.The results showed that half lives of bcl-2 and GAPDH mRNA were 6.13 hours and over 20 hours respectively in sinusoidal endothelial cells,and 5.86 hours and 13.74 hours respectively in Kupffer cells. |
| Key words: RT-PCR sinusoidal endothelial cells Kupffer cells mRNA half life determination |
