| 摘要: |
| 应用还原和非还原单向SDS-PAGE及二者结合的双向电泳技术,研究了红苋R104种子谷蛋白的亚基组成。谷蛋白由多种亚基组成,高分子量二硫键连接蛋白占其多数,经还原裂解成A(54 KD)、B(40 KD,39 KD)、C(33 KD,31 KD)、D(22 KD,20 KD,18 KD,)和E(15 KD)5组主要单肽链单元,提出了二硫键连接蛋白的组成模式,并发现低分子量谷蛋白亚基存在肽内二硫键。 |
| 关键词: 籽粒苋，谷蛋白，双向电泳，二硫键连接蛋白 |
| DOI： |
| 分类号:Q512.4 |
| 基金项目:国家自然科学基金 |
|
| Disulphide-linked Proteins and Subunit Composition of the Glutelin of Amaranth credentus R104 |
|
Song Hongxin,Song Hongxin
|
| Abstract: |
| Under non-reducing or reducing conditions,the onedimensional or two dimensional methods were employed in this research on the glutelin of A. credentus R104 seed.The results showed that the glutelin consisted of many subunits.The majority of the glutelin was HMW disulphide-linked proteins,which could be dissociated into five groups by reducing: A(54 KD), B(40 KD, 39 KD),C(33 KD,31 KD),D(22 KD,20 KD,18 KD),and E(15 KD).So the HMW protein composition model was put forward accordingly. |
| Key words: grain amaranth, seed protein, glutelin, electrophoresis,disulphide-linked proteins |
