| 摘要: |
| 【目的】通过甲基磺酸乙酯（EMS）突变体库系统筛选耐盐黄瓜突变体，为黄瓜耐盐机制研究和品种改 良提供种质资源。【方法】以野生型黄瓜HS9320及28份黄瓜突变体为试验材料，利用NaCl溶液（100 mmol/L）模拟盐胁迫处理，通过分析生长指标和可溶性糖、可溶性蛋白、脯氨酸、丙二醛含量和抗氧化酶（SOD、POD、CAT）活性等指标筛选耐盐突变体。【结果】盐胁迫处理下，幼苗期28份黄瓜突变体的生长表现出不同程度的变化，高耐盐型突变体M324-2-1和M369-6-2地上部和地下部鲜质量的降幅均小于野生型黄瓜HS9320，地上部和地下部鲜质量分别较对照下降31.18%，19.94%和22.07%，25.05%，叶片可溶性蛋白含量、SOD和CAT活性与对照相比均有所升高，而盐 敏感型突变体M391-1-1的叶片可溶性蛋白含量和POD活性与对照相比显著降低，这说明耐盐黄瓜突变体会通过积 累渗透调节物质，提高抗氧化酶活性以抵御盐胁迫的损伤。【结论】通过黄瓜突变体幼苗期耐盐性试验，共筛选到4份高耐盐型材料和9份耐盐型材料，分别为M324-2-1、M369-6-2、M112-6-1、M68-3-2及M59-8-1、M7-6-1、M30-5-1、 M389-9-1、M378-6-2、M324-2-3、M132-7-1、M378-6-3和M9-2-2。 |
| 关键词: 黄瓜 甲基磺酸乙酯（EMS） 耐盐性 突变体 |
| DOI：10. 13207/j. jnwafu. 2027. 01. 002 |
| 分类号: |
| 基金项目:吉林省科技发展计划项目（20240403004NC）；吉林省大学生创新训练计划项目（S202510193109，202410193068） |
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| Screening and identification of cucumber salt⁃tolerant mutants induced by ethyl methanesulfonate |
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Lai Xiaolin, Zhou Yanli, Cao Yu, Guan Youquan, Han Jiaqi, Luo Shihan, Sun Qiqing, Xue Wei, Meng Jingjing
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College of Horticulture，Jilin Agricultural University，Changchun，Jilin 130118，China
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| Abstract: |
| 【Objective】This study aims to systematically screen salt-tolerant cucumber mutants from the ethyl methanesulfonate（EMS） mutant library，thereby providing germplasm resources for exploring salt tolerance mechanisms and variety improvement in cucumber.【Method】The wild-type cucumber HS9320 and 28 cucumber mutants were selected as experimental materials，and salt stress was simulated with 100 mmol/L NaCl solution. Salt-tolerant mutants were screened by analyzing growth indices，contents of soluble sugar，soluble protein，proline，malondialdehyde，as well as activities of antioxidant enzymes including SOD，POD and CAT.【Result】Under salt stress，the 28 cucumber mutants at seedling stage exhibited varying degrees of growth changes. The reductions in shoot and root fresh weight of highly salt-tolerant mutants M324-2-1 and M369-6-2 were lower than those of wild-type HS9320，with their shoot and root fresh weight decreasing by 31.18% and 19.94%，22.07% and 25.05% compared with the control，respectively.These salt-tolerant mu-tants exhibited higher leaf soluble protein content as well as SOD and CAT activities than the control，whereas salt-sensitive mutant M391-1-1 showed significant reductions in leaf soluble protein content and POD activity.These findings suggest that salt-tolerant mutants can mitigate salt-induced damage by accumulating osmotic adjustment substances and increasing antioxidant enzyme activities.【Conclusion】Through salt tolerance experiments on cucumber mutant seedlings，four highly salt-tolerant and nine salt-tolerant mutants were screened，namely M324-2-1，M369-6-2，M112-6-1，M68-3-2，as well as M59-8-1，M7-6-1，M30-5-1，M389-9-1，M378-6-2， M324-2-3，M132-7-1，M378-6-3 and M9-2-2. |
| Key words: cucumber ethyl methanesulfonate（EMS ） salt-tolerance mutant |
