| 摘要: |
| 【目的】探究大田物候条件下甘蓝型冬油菜（Brassica napus L. ）应答低温胁迫时光合作用-天线蛋白相 关基因的表达及功能，为甘蓝型冬油菜抗寒品种的选育提供理论依据。【方法】以强抗寒的GZ9和弱抗寒的TSG两个甘蓝型冬油菜为试验材料，分别在适温（T1：10 ℃）-低温（T2：-4 ℃）-恢复适温（T3：20 ℃）处理下，通过转录组测 序技术研究甘蓝型冬油菜大田种植响应低温胁迫的调控途径。【结果】共鉴定出1 192个温度敏感型差异表达基因 （differentially expressed genes，DEGs），其中光合作用-天线蛋白通路富集显著 。 Lhcb6蛋白2个调控基因BnaA03G0206900ZS和BnaC03G0243700ZS在低温胁迫后显著上调，而Lhcb1、Lhcb2和Lhcb3蛋白的8个DEGs在低温胁迫下均下调。功能分析显示，Lhcb6蛋白的两个调控基因均编码稳定的亲水性蛋白，具有完整的开放阅读框，氨 基酸序列相似度达97.67%，均定位于叶绿体，为PLN00089超家族成员，与岩藻黄素-叶绿素a/c结合蛋白相关，可能参与光保护及低温适应。【 结论】甘蓝型冬油菜响应低温胁迫与岩藻黄素-叶绿素a/c结合蛋白相关基因调控有关。 |
| 关键词: 甘蓝型冬油菜 低温胁迫 转录组分析 光合作用-天线蛋白 Lhcb6 蛋白 差异表达基因 |
| DOI：10. 13207/j. jnwafu. 2026. 11. 011 |
| 分类号: |
| 基金项目:甘肃省高校教师创新基金项目（2024B-173）；庆阳市青年科技人才项目（2025RK1030）；甘肃省科技计划项目（24JRRM025）； 陇东学院青年博士基金项目（XYBYZK2209） |
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| Expression and functional analysis of photosynthesis⁃antenna protein⁃related genes in Brassica napus under low temperature stress |
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JIN Jiaojiao1, LIU Haiqing1, REN Panrong1, WANG Chunlin1, SUN Wancang2
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1.School of Agriculture and Bioengineering，Longdong University/Gansu Key Laboratory of Conservation and Utilization of Biological Resources and Ecological Restoration in Longdong，Qingyang，Gansu 745000，China;2.Agronomy College，Gansu Agriculture University/Gansu Rapeseed Engineering Research Center，Lanzhou，Gansu 730000，China
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| Abstract: |
| 【Objective】This study investigated the expression and function of photosynthetic-antenna pro-tein-related genes in Brassica napus L. in response to low-temperature stress under field phenological condi-tions，to provide a theoretical basis for breeding cold-resistant varieties of Brassica napus L. .【Method】Using two rapeseed-type winter oilseed rape cultivars，namely GZ9（high cold resistance） and TSG（low cold resis?tance），as experimental materials，this study investigated the transcriptional regulatory pathways underlying the cold stress response of field-grown rapeseed-type winter oilseed rape through transcriptome sequencing technol-ogy under three treatments：optimal temperature（T1：10 °C），low temperature（T2：-4 °C），and recovery to optimal temperature（T3：20 °C）.【Result】A total of 1 192 temperature-sensitive differentially expressed genes （DEGs） were identified，with significant enrichment in the photosynthesis-antenna protein pathway. Two regu-latory genes of Lhcb6 protein，BnaA03G0206900ZS and BnaC03G0243700ZS，showed significant upregulation under low-temperature stress，while eight DEGs of Lhcb1，Lhcb2，and Lhcb3 proteins were downregulated.Functional analysis revealed that both regulatory genes of Lhcb6 encode stable hydrophilic proteins with intact open reading frames，exhibiting 97.67% amino acid sequence similarity. These proteins are localized in chloro?plasts，belonging to the PLN00089 superfamily，and are associated with fucoxanthin-chlorophyll a/c-binding pro-teins，suggesting their potential involvement in light protection and low-temperature adaptation.【Conclusion】The response of winter rapeseed（Brassica napus L.）to low-temperature stress is associated with the regulation of genes related to fucoxanthin-chlorophyll a/c binding protein. |
| Key words: winter rapeseed（Brassica napus） low temperature stress transcriptome analysis photosynthe‑sis-antenna proteins Lhcb6 protein differentially expressed genes |
