| 摘要: |
| 【 目的】解析绵羊肌苷酸（inosine monophosphate，IMP）代谢相关基因及通路，为绵羊肉品质相关的IMP调控研究提供参考。【 方法】以杜泊羊、滩羊、小尾寒羊为研究对象，采集其背最长肌样品，测定其IMP和胆固醇的含量。构建羊cDNA文库，进行转录组测序，筛选差异表达mRNAs（DE mRNAs）和差异表达lncRNAs（DE ln?cRNAs），预测DE lncRNAs的靶基因，对DE mRNAs和DE lncRNAs的靶基因进行GO和KEGG富集分析。对DE mRNAs与DE lncRNAs进行互作分析。采用RT-qPCR方法检测相关DE mRNAs和DE lncRNAs的转录水平，以验证测序数据的可靠性。【 结果】3个绵羊品种背最长肌胆固醇含量差异不显著；杜泊羊IMP含量显著低于滩羊和小尾 寒羊（P<0.05），后二者间无显著差异。在杜泊羊与滩羊、杜泊羊与小尾寒羊的比较中选出差异表达mRNA和ln?cRNA，两组取交集后获得232个DE mRNAs和278个DE lncRNAs。GO富集分析结果显示，DE mRNAs显著富集于92个功能条目，其中包括半胱氨酸型内肽酶抑制剂活性等；KEGG分析结果表明，DE mRNAs 参与159条通路，其中包括色氨酸代谢、TNF信号通路和亚油酸代谢等可能与IMP代谢相关的通路。对DE lncRNAs的靶基因进行预测，共得到1 657个靶基因（803个反式靶基因、854个顺式靶基因）。DE lncRNA反式和顺式靶基因分别显著富集于129和137个GO条目，其中包括rRNA加工、核糖体生物合成和细胞内信号转导等，并且分别富集到228和226条KEGG通路，其中包括RIG-I样受体信号通路、MAPK信号通路、催乳素信号通路等。这些信号通路和生物学过程涉及多个与 肌肉发育和IMP代谢密切相关的基因。对6个DE mRNAs和6个DE lncRNAs进行RT-qPCR验证，结果与转录组测序结果一致，表明转录组测序结果是可靠的。【 结论】试验揭示了绵羊背最长肌的DE mRNAs和DE lncRNAs及相关信号通路，筛选了部分基因（IGFBP7、CRB1、NRG4等）及lncRNA（TCONS_00048011、TCONS_00062426、TCONS_ 00039964、TCONS_00076277等）可能与绵羊IMP代谢调控相关。 |
| 关键词: 绵羊 肉品质 肌苷酸 转录组测序 mRNA lncRNA |
| DOI：10. 13207/j. jnwafu. 2026. 09. 010 |
| 分类号: |
| 基金项目:宁夏自然科学基金项目（2020AAC03082）；宁夏回族自治区农业育种专项（nxnyyz20150101） |
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| Screening of IMP metabolism⁃related genes in sheep based on RNA⁃seq |
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WANG Dingxiang, LI Chenglong, NIE Wei, MENG Ke, FENG Dengzhen, KANG Xiaolong
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Key Laboratory of Molecular and Cellular Breeding of Ruminants in Ningxia Hui Autonomous Region，College of Animal Science and Technology，Ningxia University，Yinchuan，Ningxia 750021，China
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| Abstract: |
| 【 Objective】This research aims to analyze genes and pathways related to inosine monophosphate （IMP） metabolism in sheep，to provide a reference for IMP regulation studies concerning sheep meat quality. 【Method】With Dorper sheep，Tan sheep，and Small-tailed Han sheep as research subjects，samples of their lon-gissimus dorsi muscle were collected，and their IMP and cholesterol levels were measured. A cDNA library was constructed for transcriptome sequencing to screen differentially expressed mRNA（DE mRNAs） and defferentially expressed long non-coding RNAs（DE lncRNAs）. Target gene of DE lncRNAs were predicted，and GO and KEGG enrichment analysis were performed on DE mRNAs and the target genes of DE lncRNAs. Interaction analysis was conducted on DE mRNAs and DE lncRNAs. RT-qPCR was used to detect the transcription levels of relevant DE mRNAs and DE lncRNAs，thereby validating the reliability of the sequencing data.【Result】There were no significant differences in cholesterol content in longissimus dorsi muscles among the three sheep breeds. However，the IMP content in Dorper sheep was significantly lower than those in Tan sheep and Small-tailed Han sheep（P<0. 05），while no significant difference was observed between Tan sheep and Small-tailed Han sheep. DE mRNAs and DE lncRNAs were selected from comparisons between Dorper and Tan sheep，and between Dorper and Small-tailed Han sheep.The intersection of these two groups contained 232 DE mRNAs and 278 DE lncRNAs.GO enrichment analysis revealed that DE mRNAs were significantly enriched in 92 functional entries，including cysteine-type endopeptidase inhibitor activity. KEGG analysis indicated that DE mRNAs were involved in 159 pathways，including tryptophan metabolism，TNF signaling pathway，and linoleic acid metabolism，which were potential pathways related to IMP metabolism. Target gene prediction for DE lnc-RNAs yielded 1 657 target genes（803 trans-target genes and 854 cis-target genes）.Enrichment analysis revealed that trans-and cis-acting factors of DE lncRNA were significantly enriched in 129 and 137 GO entries，respectively，including rRNA processing，ribosome biogenesis，and intracellular signal transduction. They were also enriched in 228 and 226 KEGG pathways，respectively，encompassing RIG-I-like receptor signaling pathway，MAPK signaling pathway，and prolactin signaling pathway. These pathways and biological processes involve multiple genes closely related to muscle development and IMP metabolism. RT-qPCR validation of 6 DE mRNAs and 6 DE lncRNAs produced results consistent with the transcriptomic sequencing results，demonstrating the reliability of the sequencing data.【Conclusion】This study identified the DE mRNAs and DE lncRNAs in sheep longissimus dorsi muscle and the associated signaling pathways. Certain genes （IGFBP7，CRB1，NRG4，etc.） and lncRNAs （TCONS_00048011，TCONS_00062426，TCONS_00039964，TCONS_00076277，etc.）were screened as potential candidates involved in sheep muscle IMP metabolic regulation. |
| Key words: sheep meat quality IMP RNA-seq mRNA lncRNA |
