| 摘要: |
| 【 目的】探究TaMKK9在小偃6号高温抗条锈病过程中发挥的功能，揭示MAPK级联参与小麦高温抗条锈病的分子机理，完善小麦高温抗病调控网络。【 方法】在烟草叶片中进行TaMKK9亚细胞定位；利用病毒诱导的基因沉默和病毒诱导的基因过表达，研究TaMKK9在小麦高温抗条锈病中发挥的作用；采用实时荧光定量PCR，检测TaMKK9及抗病相关基因的表达量；通过酵母双杂交筛选TaMKK9下游互作靶标，并运用双分子荧光互补（BiFC）、免疫共沉淀（Co-IP）及GST Pull-down试验验证TaMKK9与TaMPK3的互作关系。【 结果】TaMKK9是定位于细胞膜、细胞质和细胞核的蛋白激酶。实时荧光定量PCR结果表明 ，受条锈菌和高温双重诱导 ，小偃6号TaMKK9的表达上调5.17倍；与对照组相比，沉默TaMKK9的小偃6号植株接种条锈菌后，叶片表面的条锈菌夏孢子堆显著增多，防御相关基因TaPR1的表达降低66.9%~94.4%，转高温24和48 h后叶片H2O2积累量分别下降29.9%和59.5%；过表达TaMKK9的小偃6号植株接种条锈菌后，条锈菌生物量降低60.5%~72.0%，小麦植株抗病性显著增强。TaMKK9与TaMPK3互作验证结果表明，TaMKK9与TaMPK3存在互作。【 结论】在小偃6号高温抗条锈病过程中，受条锈菌和高温双重诱导，导致TaMKK9表达量显著增加。TaMKK9与下游TaMPK3互作传递免疫信号，参与ROS信号通路，通过影响小麦植株H2O2的积累正调控小麦植株对条锈菌的抗性。 |
| 关键词: 条锈菌 小偃6号 高温抗条锈性 MAPK |
| DOI：10. 13207/j. jnwafu. 2026. 06. 007 |
| 分类号: |
| 基金项目:科技部国际合作项目（KY202002018，G2023172013L）；国家现代农业（小麦）产业技术体系项目（CARS-03-37）；国家自然科学基金项目（31972219）；西北农林科技大学推广项目（TGZX2021-13）；西北农林科技大学高水平创新团队项目（XYTD2023-04） |
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| Study on TaMKK9 in regulating the high-temperature resistance of wheat cultivar Xiaoyan 6 to stripe rust |
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FENG Junjia, LIU Yuyang, LI Hai, HU Xiaoping
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Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education/Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs，College of Plant Protection，Northwest A&F University，Yangling，Shaanxi 712100，China
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| Abstract: |
| 【 Objective】This study aims to explore the function of TaMKK9 in the process of high-tempera-ture induced stripe rust resistance in Xiaoyan 6，and to elucidate the molecular mechanism underlying the involvement of the MAPK（mitogen-activated protein kinase） cascade in wheat’s high-temperature induced stripe rust resistance，in order to refine the regulatory network for disease resistance in wheat under high-temperature conditions.【Method】Virus-induced gene silencing and virus-induced gene overexpression were used to explore the role of TaMKK9 in wheat’s resistance to stripe rust under high-temperature conditions. Real-time fluorescence quantitative PCR was used to detect the expression levels of TaMKK9 and other genes related to disease resistance. Downstream interaction targets of TaMKK9 were screened by yeast two-hybridization，and the interaction relationship was verified by BiFC，Co-IP，and GST Pull-down.【Result】TaMKK9 was a protein kinase loca-lized to the cell membrane，cytoplasm，and nucleus. Real-time quantitative PCR validation showed that the expression level of TaMKK9 in Xiaoyan 6 was upregulated by 5. 17 times，which was induced by stripe rust and
high temperature. Compared to the control，Xiaoyan 6 plants with TaMKK9 silenced exhibited more uredinium on leaf surfaces after stripe rust inoculation，accompanied by a 66.9%?94.4% reduction in the expression of the defense-related gene TaPR1.After exposure to high temperature for 24 and 48 hours，H2O2 accumulation in leaves decreased by 29.9% and 59.5%，respectively. Overexpression of the TaMKK9 gene in Xiaoyan 6 plants resulted in a 60.5%?72.0% reduction in stripe rust fungus biomass and significantly enhanced disease resis?tance in wheat plants. The interaction verification results between TaMKK9 and TaMPK3 indicated that TaMKK9 interacted with the downstream TaMPK3.【Conclusion】 During the resistance process against stripe rust in Xiaoyan 6 under high-temperature conditions，the expression level of TaMKK9 increased due to dual induction by stripe rust and temperature. TaMKK9 interacted with the downstream TaMPK3 to transmit immune signals and positively regulates the resistance of wheat plants to stripe rust by affecting H2O2 accumulation in plants. |
| Key words: stripe rust Xiaoyan 6 high temperature resistance to stripe rust MAPK |
