| 摘要: |
| 目的】探讨豆瓣菜对Cd胁迫的分子响应机制，为减轻豆瓣菜Cd危害和促进植物抗重金属研究提供 参考。【 方法】以水培豆瓣菜幼苗为材料，设置0，0. 2，0. 8，3. 2，12. 8 mg/L CdCl2处理，筛选适宜Cd胁迫质量浓度，并考察其对相关生长生理指标、代谢物含量及基因表达的影响。【 结果】①在适宜质量浓度（0. 8 mg/L CdCl2）Cd胁迫 下，豆瓣菜植株的生物量和光合作用受到显著抑制，S含量显著减少，而Cd、Fe、Mn、K和Ca含量显著增加；POD和SOD活性显著下降，而MDA含量显著上升；根部维管束面积减小，细胞壁增厚及胞内质体、线粒体受损。②Cd胁迫下调 MAPK 信号通路相关基因（133个）和苯丙烷类合成途径相关基因（104个）表达，抑制应激响应和细胞壁强化；同 时抑制糖酵解/糖异生途径基因表达，影响能量代谢；但同时促进过氧化物酶体相关基因表达上调，减轻氧化损伤。 ③Cd胁迫抑制植株糖酵解及淀粉和蔗糖代谢途径相关代谢物合成。【 结论】0. 8 mg/L CdCl2胁迫显著抑制豆瓣菜的 生长和光合作用。Cd通过抑制抗氧化系统活性及碳代谢通路相关酶基因的表达，影响碳代谢产物的积累，最终阻碍 豆瓣菜的生长。 |
| 关键词: 豆瓣菜 镉胁迫 糖酵解/糖异生途径 过氧化物酶体途径 |
| DOI：10. 13207/j. jnwafu. 2026. 05. 007 |
| 分类号: |
| 基金项目:农业农村部申请保护品种 DUS 测试及已知品种库维护项目（h20230603）；广东省农业科学院创新基金-产业专项“纳米二硫化钼降低芥菜镉吸附机制研究”（202230） |
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| Multi⁃omics analysis of watercress response to cadmium stress |
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LAI Zixin1, TAN Wanxiang1,2, LUO Shanwei2, LI Guihua2, XU Zhenjiang1, JIANG Yuan1
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1.College of Agriculture，South China Agricultural University/Guangzhou Sub-center for New Plant Variety Tests，Ministry of Agriculture and Rural Affairs，Guangzhou，Guangdong 510642，China;2.Vegetable Research Institute，Guangdong Academy of Agricultural Sciences，Guangzhou，Guangdong 510640，China
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| Abstract: |
| 【 Objective】The study aims to explore the mechanisms underlying watercress（Nasturtium officinale） response to Cadmium（Cd） stress，to provide a reference basis for mitigating Cd toxicity and advancing research on plant resistance to heavy metals.【Method】Hydroponically grown watercress seedlings were treated with 0，0.2，0.8，3.2， and 12.8 mg/L CdCl2 to determine the appropriate Cd concentration. The effects of Cd stress on growth， physiological indices，metabolite levels，and gene expression were investigated.【Result】① Under 0. 8 mg/L CdCl2 stress，biomass and photosynthesis of watercress were significantly inhibited. Sulfur（ S） content was decreased，while Cd，Fe，Mn，K，and Ca contents were increased. Activities of POD and SOD were declined，whereas MDA levels were increased. Additionally，root vascular bundle area was decreased，cell walls were thickened，and intracellular structures，such as plastids and mitochondria，were damaged. ② Cd stress downregulated the expression of the genes associated with the MAPK signaling pathway（133 genes） and phenylpropanoid biosynthesis pathway（104 genes），impairing stress responses and cell wall reinforcement. It also suppressed the expression of genes related to glycolysis and gluconeogenesis，affecting energy metabolism，while simultaneously upregulating the expression of genes involved in peroxisome activity，alleviating oxidative damage. ③ Cd stress inhibited the synthesis of metabolites related to glycolysis，starch，and sucrose metabolism.【Conclusion】0.8 mg/L CdCl2 stress significantly inhibited the growth and photosynthesis of watercress. Transcriptomic and metabolomic analyses showed that Cd affected the accumulation of carbon metabolites by inhibiting the activity of the antioxidant system and the expression of genes related to the carbon metabolism pathway，ultimately hindering the growth of watercress. |
| Key words: watercress Cd stress glycolysis/gluconeogenesis peroxisome |
