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码上扫一扫！ |
| 丝藻光合生理、抗氧化系统及细胞营养对 盐胁迫的响应 |
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孙文志1,2,3, 蒲亚雪2,3, 杨宋琪2,3, 王杰1,2,3, 孙小妹1, 罗光宏1,2,3
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1.甘肃农业大学 资源与环境学院,甘肃 兰州 730070;2.河西学院 甘肃省微藻技术创新中心,河西走廊资源利用重点实验室,甘肃 张掖 734000;3.甘肃凯源生物技术开发中心有限责任公司,甘肃 张掖 734000
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| 摘要: |
| 【目的探究丝藻生长、光合活性、抗氧化系统和细胞营养对盐胁迫的响应,为丝藻应用于盐渍化土壤及咸水生态修复、高盐污水处理等领域提供数据支撑。【方法】以 NaCl为盐胁迫因子,设置6个质量浓度梯度(0,2,4,8,16,32 g/L)培养丝藻21 d,测定其光合生理、抗氧化酶活性及细胞营养等指标。【结果】NaCl质量浓度在0~16 g/L时,藻密度随着培养时间增加而增加;丝藻最大比生长速率及部分光合参数随 NaCl质量浓度升高而显著降低;4~8 g/L NaCl质量浓度下丝藻 CAT活性、SOD活性、MDA 含量均显著增加,而32 g/L NaCl质量浓度下其抗氧化酶活性均显著降低;丝藻类胡萝卜素、多糖、蛋白质及油脂含量随 NaCl质量浓度增加而显著增加,尤其在高盐环境中其油脂含量较对照提高50%~105%。【结论】盐胁迫条件下丝藻生长及光合活性受到一定程度抑制,但在逆境中其抗氧化酶活性及细胞营养呈现规律性变化,以提高自身抗逆性,进而更好地适应盐胁迫环境。 |
| 关键词: 丝藻 盐胁迫 光合生理 抗氧化酶活性 细胞营养 |
| DOI：10.13207/j.jnwafu.2026.04.018 |
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| 基金项目:甘肃省科技计划项目(22YF7FG188);甘肃省高等学校青年博士项目(2024QB-110);河西学院博士科研启动金项目(KYQD2024010) |
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| Responses of photosynthetic physiology,antioxidant system,and cellular nutrition of Ulothrix to salt stress |
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SUN Wenzhi1,2,3, PU Yaxue2,3, YANG Songqi2,3, WANG Jie1,2,3, SUN Xiaomei1, LUO Guanghong1,2,3
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1.College of Resources and Environment,Gansu Agricultural University,Lanzhou,Gansu 730070,China;2.Gansu Microalgae Technology Innovation Center,Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Hexi University,Zhangye,Gansu 734000,China;3.Gansu Kaiyuan Biotechnology Development Center Co.,Ltd.,Zhangye,Gansu 734000,China
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| Abstract: |
| 【Objective】The study aims to explore the responses of growth,photosynthetic activity,antioxidant system,and cellular nutrition of Ulothrix to salt stress,and to provide data for the application of Ulothrix in the ecological restoration fields of the salinized soil and brackish water as well as in the highsalt sewage treatment.【Method】Six concentrations (0,2,4,8,16,32 g/L) were set with NaCl as the salt stress factor.Ulothrix was incubated for 21 days to determine its photosynthetic physiology,antioxidant enzymes,and cellular nutrition.【Result】Algal density was increased with time at NaCl concentrations ranging from 0 to 16 g/L.The maximum specific growth rate and some photosynthetic parameters were decreased
with increasing NaCl concentration.The activities of CAT activity,SOD activity and MDA content increased significantly at 4-8 g/L NaCl concentration,while the activity of antioxidant enzymes decreased significantly at 32 NaCl concentration.Carotenoid,polysaccharides,proteins,and oil contents were increased with increasing NaCl concentration,especially the oil content was 50% to 105% higher than the control at high salt.【Conclusion】The growth
and photosynthetic activity of Ulothrix are inhibited under salt stress,but the antioxidant enzyme activity and cellular nutrition change to improve resistance,allowing for better adaptation to a salt-stressed environment. |
| Key words: Ulothrix salt stress photosynthetic physiology antioxidant enzyme cellular nutrients |
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