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码上扫一扫！ |
| 西瓜突变体库中耐盐材料的筛选与鉴定 |
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岳贞1,2, 郭宸硕2, 侯尹婕2, 尹丽娟2, 杨万邦1, 代雪2, 魏春华2, 于蓉1
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1.宁夏农林科学院 园艺研究所,宁夏 银川 750002;2.西北农林科技大学 园艺学院,陕西 杨凌 712100
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| 摘要: |
| 【目的】对西瓜突变体库中耐盐材料进行筛选与鉴定,为西瓜耐盐种质选育及耐盐机制的研究提供依据。【方法】首先以西瓜种质M08为材料,通过NaCl浓度梯度(0(CK),250,270,290,310和330 mmol/L)试验,确定西瓜种子萌发期盐胁迫最适浓度;然后在 M08的突变体库中随机挑选23份M2代种子,并且以M08种子为对照,以筛选的最适 NaCl浓度进行盐胁迫处理,测定种子的生长指标,从中筛选出可种子萌发的株系;最终挑选了NO.55和NO.60中长势较为正常的幼苗,以 M08幼苗为对照,采用盆栽法,以350 mmol/L NaCl溶液进行灌根处理,观察西瓜幼苗的表型,并测定叶片的脯氨酸含量和过氧化物酶(POD)活性,筛选出耐盐性较强的西瓜突变体材料。【结果】与CK相比,不同浓度的NaCl处理后,M08种子的发芽数、发芽势、发芽率、发芽指数均明显降低,种子萌发期盐胁迫的NaCl最适浓度为310 mmol/L。在种子萌发期,从23份西瓜M2代种子中共筛选出10份种子可萌发的株系,进一步结合生长指标综合评判,其中7份株系的耐盐性优于对照。用350 mmol/L NaCl溶液进行灌根处理3 h后,CK和NO.55幼苗表现出明显的萎蔫,叶片下垂或卷曲;而NO.60幼苗仍未发生明显变化,且其叶片的脯氨酸含量和过氧化物酶活性显著高于CK和NO.55。【结论】通过种子萌发期和幼苗生长期耐盐试验,在 M08突变体库中成功鉴定了耐盐性突出的材料 NO.60,为后续西瓜耐盐性改良提供种质资源。 |
| 关键词: 西瓜 突变体库 NaCl胁迫 耐盐性评价 |
| DOI：10.13207/j.jnwafu.2026.04.009 |
| 分类号: |
| 基金项目:宁夏农林科学院高质量发展和生态保护科技创新示范项目(NGSB-2021-7);国家西甜瓜产业技术体系专项(CARS-25) |
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| Screening and Identification of the salt-tolerant materials of watermelon mutant library |
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YUE Zhen1,2, GUO Chenshuo2, HOU Yinjie2, YIN Lijuan2, YANG Wanbang1, DAI Xue2, WEI Chunhua2, YU Rong1
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1.Institute of Horticulture,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002,China;2.College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China
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| Abstract: |
| 【Objective】Screening and dentification of salt-tolerant materials from the watermelon mutant library were conducted to provide a basis for the breeding of salt-tolerant watermelon germplasm and the investigation of salt-tolerant mechanisms.【Method】First,with watermelon germplasm M08 as the material,the optimal concentration of NaCl for salt stress during the seed germination stage was determined through NaCl concentration gradient (0 (CK),250,270,290,310 and 330 mmol/L)
tests.Then,23 seeds of M2 generation were randomly selected from the mutant library of M08,and salt stress treatment was conducted under the optimal NaCl concentration selected with M08 seeds as the control.The growth indicators of the seeds were measured,and the lines with germinable seeds were screened out.Given that seedlings of relatively normal growth were only observed in NO.55 and NO.60 lines among all the selected lines with germinable seeds,seedlings from
NO.55 and NO.60 strains were used as experimental materials,with M08 seedlings as the control.The pot method was adopted,and the seedlings were treated with 350 mmol/L NaCl solution for root irrigation.The phenotype of the watermelon
seedlings was observed,and the proline content and the activity of peroxidase (POD) in the leaves were measured to screen out watermelon mutant materials with strong salt tolerance.【Result】Compared with CK,the number of germinated seeds,germination potential,germination rate,and germination index of M08 seeds were all significantly reduced after treatment with different concentrations of NaCl.The optimal NaCl concentration for salt stress during the seed germination
stage was 310 mmol/L.During the seed germination stage,10 lines with germinable seeds were selected from 23 watermelon seeds of M2 generation.Further comprehensive evaluation based on growth indicators showed that the salt tolerance of 7 lines was better than that of the control.After 3 hours of root irrigation treatment with 350 mmol/L NaCl solution,the seedlings of CK and NO.55 showed obvious wilting,with leaves drooping or curling,while the seedlings of NO.60 showed no significant changes. Moreover,the proline content and peroxidase activity in the leaves of NO.60 were significantly higher than those of CK and NO.55.【Conclusion】Through salt tolerance tests conducted during the seed germination and seedling
growth stages,a mutant line with exceptional salt tolerance,namely NO.60,was successfully identified from the M08 mutant library,which provided valuable germplasm resources for the improvement of salt tolerance in watermelon. |
| Key words: watermelon mutant library NaCl stress evaluation of salt tolerance |
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