| 摘要: |
| 【目的通过构建NtCET4基因过表达植株，分析该基因在调控烟草开花时间中的作用，探讨基于NtCET4操纵提高烟草有效叶数量从而提高烟叶产量的可行性。【方法】构建NtCET4基因过表达载体后，通过农杆菌介导的叶盘转化法转化烟草获得转化植株，PCR和GUS染色鉴定的转化植株通过苗期RT-qPCR分析过表达特性，过表达株系移栽后观察其开花时间、有效叶数量的变化，并通过RT-qPCR分析NtCET4在开花前后相对表达量的变化。【结果】与野生型‘K326’相比，9个转基因株系中4个株系为NtCET4过表达株系。NtCET4过表达株系花芽分化时间显著延迟(较‘K326’晚12 d左右)，有效叶片数量增加。检测开花前后的NtCET4表达量发现，过表达株系的表达量均高于野生型，且开花前表达量大于开花后。【结论】烟草植株中NtCET4过表达能有效延长营养芽活动时间从而实现延迟开花，增加植株的有效叶片数量。 |
| 关键词: 烟草 NtCET4基因 开花时间 有效叶数量 |
| DOI：10.13207/j.cnki.jnwafu.2025.12.003 |
| 分类号: |
| 基金项目:贵州省科技厅项目(Z073181) |
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| Effet of overexpression of NtCET4 gene on tobacco vegetative buds and delayed flowering |
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LONG Junlan1,2, LUO Rui1, LUO Xu1,2, ZHANG Lan1,2
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1.College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;2.Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education),Guizhou University,Guiyang,Guizhou 550025,China
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| Abstract: |
| 【Objective】The effect of NtCET4 gene overexpression in regulating tobacco flowering time was analyzed by constructing NtCET4 overexpression plants,and the feasibility of increasing the number of effective leaves of tobacco to improve tobacco yield based on NtCET4 manipulation was discussed.【Method】Overexpression vector of NtCET4 was constructed.Transgenic individuals were obtained through the Agrobacterium-mediated leaf disc transformation.The transgenic plants were identified by PCR and GUS staining.The overexpressed lines were selected from transgenic lines based on RT-qPCR.The overexpressed plants were transplanted into fields to observe flowering time and leaf numbers.RT-qPCR was used to identify relative expressions of NtCET4 before and after flowering.【Result】During the seedling stage,four transgenic lines showed significant increase in NtCET4 expression.These four transgenic lines with NtCET4 overexpression showed delaying flowering (about 12 days).Longer period of vegetative bud activity resulted in more valid leaves in the transgenic plants.NtCET4 expression in transgenic plants was increased from seedling to mature,and higher expression was detected before flowering relative to after flowering.【Conclusion】Overexpression of NtCET4 promotes the vegetative bud activity,delays flowering,and increases the number of valid leaves in tobacco. |
| Key words: tobacco NtCET4 gene flowering time number of valid leaves |
