| 摘要: |
| 【目的】解析牛肌肉发育过程中超级增强子、潜在靶基因及转录因子的调控机制。【方法】利用牛原代成肌细胞H3K27ac和H3K4me1 2种组蛋白修饰的ChIC-seq数据，分析和鉴定牛原代成肌细胞基因组超级增强子。利用ROSE软件注释超级增强子的靶基因，并对其进行GO和KEGG分析。对超级增强子区域进行基序富集分析。【结果】利用H3K27ac标记鉴定得到295个超级增强子，利用H3K4me1标记鉴定出362个超级增强子，其中二者共有超级增强子为143个。GO和KEGG分析表明，共有超级增强子调控的潜在靶基因主要富集在黏着斑、PI3K-Akt和病毒感染等信号通路，这些通路在调控成肌细胞增殖、分化和肌纤维形成中发挥着关键作用。对共有超级增强子区域进行基序富集分析，筛选并确定了MYB70和HLF等肌肉发育关键转录因子。【结论】利用2种组蛋白修饰筛选并鉴定出牛原代成肌细胞基因组内的超级增强子，为解析牛肌肉发育过程中超级增强子的结构、功能及调控机制提供了理论基础。 |
| 关键词: 牛原代成肌细胞 H3K27ac H3K4me1 超级增强子 |
| DOI：10.13207/j.cnki.jnwafu.2025.10.001 |
| 分类号: |
| 基金项目:陕西省科技厅秦创原引进高层次创新创业人才项目(QCYRCXM-2022-26);教育部高校基本科研业务费专项资金(Z1013023005);农业部生物育种专项(2023ZD0405003) |
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| Characteristics analysis of super enhancers of bovine primary muscle cells |
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YUAN Mengli1, SONG Linjie1, SUN Shengjie1, LIU Junxia2, REN Gang1
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1.College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China;2.College of Life Science,Northwest A&F University,Yangling,Shaanxi 712100,China
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| Abstract: |
| 【Objective】This study provided a theoretical basis for understanding the regulatory mechanisms of super enhancers,putative target genes and transcription factors during bovine muscle cells development.【Method】ChIC-seq data of H3K27ac and H3K4me1 were successfully applied to identify SEs in primary bovine muscle cells.ROSE software was used to annotate the target genes of SEs and GO and KEGG analyses on these genes were subsequently conducted.Furthermore,motif enrichment analysis was performed on the regions of super-enhancers.【Result】295 SEs were identified by H3K27ac mark，while 362 were identified by H3K4me1 mark,among which 143 SEs were marked by both histone modifications.GO and KEGG analyses showed that the putative target genes,regulated by shared SEs,mainly involved in focal adhesion,PI3K-Akt signaling and viral infection pathways,which played crucial roles in regulating muscle cell proliferation,differentiation,and myofiber formation processes.Through further enrichment analysis of transcription factor binding motifs in shared SEs regions,MYB70 and HLF were screened out and identified as key transcription factors during muscle development.【Conclusion】This study identified the distribution of super enhancers with two histone modification approaches,laying a theoretical foundation for deciphering the structure,function,and regulatory mechanisms of super enhancers during bovine muscle development. |
| Key words: bovine primary muscle cell H3K27ac H3K4me1 super enhancer |
