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| 基于高通量测序筛选鸽子下丘脑和卵巢组织早熟性状相关基因 |
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蔡倩楠1,2, 施文莉1,2, 赵邦哲1,2, 李丽2, 章琳俐2, 辛清武2, 缪中纬2, 朱志明2, 陈孝秋3, 吴进伟3, 郑嫩珠2
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1.福建农林大学 动物科学学院，福建 福州 363000;2.福建省农业科学院 畜牧兽医研究所，福建 福州 350013;3.福建集盛鸽业股份有限公司，福建 泉州 362000
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| 摘要: |
| 【目的】解析调控鸽子早熟性状的关键基因和信号通路，丰富鸽子早熟性状研究数据。【方法】选取同一批次180日龄开产和未开产大宝鸽各4只，处死后采集下丘脑和卵巢组织进行高通量测序分析，筛选与鸽子早熟性状相关的差异表达基因(differentially expressed genes,DEGs)，并对其进行GO和KEGG富集分析。从DEGs中选取P值由小到大的前1 000个基因进行蛋白互作(protein-protein interaction,PPI)分析，筛选早熟性状相关DEGs。采用qRT-PCR方法检测早熟相关DEGs的转录水平，验证高通量测序数据的可靠性。【结果】①下丘脑和卵巢共鉴定出 685个DEGs。相比于未开产鸽子，已开产鸽子下丘脑中存在上调基因159个，下调基因201个；卵巢中存在上调基因118个，下调基因207个。②GO富集分析发现，DEGs显著富集于微管运动、神经系统过程、膜筏、细胞死亡和免疫反应等；KEGG分析显示，DEGs在ECM-受体相互作用、细胞因子 细胞因子受体相互作用、焦点黏连、MAPK信号通路、肌动蛋白细胞骨架调控等信号通路显著富集。这些信号通路和生物学过程涉及多个与卵泡形成和发育密切相关的基因。③筛选到AKR1D1、COL1A1、FAS、PRLR和TGFBR2等5个早熟性状相关DEGs，其中AKR1D1在开产鸽子下丘脑中上调表达，其他4个在卵巢中下调表达。④对5个DEGs进行qRT PCR验证，结果与高通量测序结果一致，表明测序结果可靠。【结论】试验揭示了开产和未开产大宝鸽下丘脑和卵巢DEGs及相关信号通路，筛选到5个可能调控鸽子早熟性状的关键基因。 |
| 关键词: 鸽子 下丘脑 卵巢 早熟性状 差异表达基因 高通量测序 |
| DOI：10.13207/j.cnki.jnwafu.2025.09.002 |
| 分类号: |
| 基金项目:泉州市专家工作站项目；“5511”协同创新工程项目（XTCXGC2021008）；福建省农业科学院科技创新团队项目（CXTD-2021006-2）；福建省星火计划项目（2023S0040） |
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| Screening genes related to precocious traits in hypothalamus and ovarian tissue of pigeons based on high-throughput sequencing |
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CAI Qingnan1,2, SHI Wenli1,2, ZHAO Bangzhe1,2, LI Li2, ZHANG Linli2, XIN Qingwu2, MIAO Zhongwei2, ZHU Zhiming2, CHEN Xiaoqiu3, WU Jinwei3, ZHENG Nenzhu2
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1.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 363000,China;2.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;3.Fujian Jisheng Pigeon Industry Co.LTD,Quanzhou,Fujian 362000,China
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| Abstract: |
| 【Objective】This research aimed to analyse the key genes and signaling pathways regulating precocious traits in pigeons to enrich the research data.【Method】A total of 8 pigeons,4 of which had laying at the age of 180 days and 4 of which had not,were selected from the same batch.The pigeons were sacrificed,and their hypothalamus and ovarian tissues were collected for high-throughput sequencing analysis.Differentially expressed genes (DEGs) related to precocious traits in pigeons were screened,and GO and KEGG enrichment analyses were performed.The top 1 000 genes with the smallest P-value from the DEGs were selected for protein-protein interaction (PPI) analysis to identify DEGs related to precocious traits.qRT-PCR was used to detect the transcription levels of the precocious-related DEGs and validate the reliability of the high-throughput sequencing data.【Result】①A total of 685 DEGs were identified in the hypothalamus and ovarian.Compared to pre-laying pigeons,159 genes were upregulated and 201 genes were downregulated in the hypothalamus of laying pigeons.118 genes were upregulated and 207 genes were downregulated in the ovarian.②GO enrichment analysis revealed that DEGs were significantly enriched in processes such as microtubule based movement,nervous system process,membrane raft,cell death,and immune response.KEGG analysis showed that DEGs were significantly enriched in signaling pathways,such as ECM receptor interaction,cytokine-cytokine receptor interaction,focal adhesion,MAPK signaling pathway,and actin cytoskeleton regulation.These signaling pathways and biological processes involved several genes closely related to follicular formation and development.③Five precocious-related DEGs were identified:AKR1D1,COL1A1,FAS,PRLR,and TGFBR2.Among them,AKR1D1 was upregulated in the hypothalamus of laying pigeons,while the other four were downregulated in the ovarian.④qRT-PCR validation of these five DEGs showed consistent results with the high-throughput sequencing data,confirming the reliability of the sequencing results.【Conclusion】This study revealed DEGs in the hypothalamus and ovarian of laying and pre-laying pigeons and identified related signaling pathways.Five key genes that may regulate precocious traits in pigeons were identified. |
| Key words: pigeon hypothalamus ovarian precocious traits differentially expressed genes high-throughput sequencing |
