| 摘要: |
| 【目的】初步探讨AcSCL4在洋葱成花中的功能，为解析洋葱成花分子调控机制提供理论基础。【方法】以长日生态型洋葱品系SB2为试验材料，根据已有的洋葱转录组数据，通过qRT-PCR筛选目的基因AcSCL4后进行基因克隆，并对其进行生物信息学分析；构建AcSCL4基因的过表达载体pB221-3300-AcSCL4并转化农杆菌，采用花序浸染法浸染拟南芥，并对T3代纯系转基因植株进行表型分析和开花相关基因表达量分析。【结果】洋葱AcSCL4基因CDS长1 515 bp，编码504个氨基酸；AcSCL4蛋白N端高度变异，C端有GRAS结构域。聚类分析发现，AcSCL4与石刁柏和棉花的SCL4蛋白亲缘关系较近。与野生型拟南芥相比，AcSCL4过表达植株表现晚花表型。荧光定量PCR结果表明，与野生型拟南芥相比,AcSCL4过表达植株开花调控基因CO和FT的表达量极显著下降。【结论】AcSCL4通过调控CO及FT基因表达来抑制洋葱开花。 |
| 关键词: 洋葱 AcSCL4 基因克隆 开花调控 |
| DOI： |
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| 基金项目:黑龙江省重点研发计划项目(GA21B012)；黑龙江省自然科学基金联合引导项目(LH2020C012) |
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| Cloning and function analysis of AcSCL4 in onion |
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ZHANG Xu,WU Xiaoxu,ZHANG Zhihui,et al
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| Abstract: |
| 【Objective】The function of AcSCL4 in flowering was preliminarily studied to provide basis for understanding molecular regulation mechanism of onion flowering regulation.【Method】AcSCL4 was identified by qRT-PCR and bioinformatics analysis based on the transcriptome data of a long-day onion inbred line SB2.The AcSCL4 overexpression vector pB221-3300-AcSCL4 was constructed and transformed to Arabidopsis by Agrobacterium tumefaciens.Homozygous Arabidopsis transgenic lines (T3) were used for further phenotypic characterization and genes expression identification.【Result】The cDNA of AcSCL4 gene was 1 515 bp and encoded 504 amino acids.AcSCL4 had conserved GRAS domain at the C terminus and was highly variable at the N terminus.AcSCL4 was phylogenetically closer to the SCL4 of Asparagus officinalis and Gossypium hirsutum.Overexpression of AcSCL4 delayed the flowering time of wild-type Arabidopsis.The expressions of CO and FT were significantly decreased in AcSCL4 overexpression transgenic lines.【Conclusion】AcSCL4 suppressed flowering by regulating CO and FT expressions. |
| Key words: onion AcSCL4 gene cloning flowering regulation |
