| 摘要: |
| 【目的】研究腐植酸（HA）对干旱胁迫下燕麦叶片光合参数与内源激素的影响，为燕麦的抗旱种植提供理论指导。【方法】以燕麦品种‘燕科2号’为材料，采用盆栽试验，分别在正常供水（田间持水量75%）、中度干旱胁迫（田间持水量45%）、重度干旱胁迫（田间持水量30%）3个水分条件下喷施HA，以喷施等量清水的处理为对照（CK），研究干旱胁迫下喷施HA后燕麦株高、单株叶面积、叶片光合参数(净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）、胞间CO2浓度（Ci）)和内源激素(生长素（IAA）、赤霉素（GA）、玉米素核苷（ZR）、脱落酸（ABA）)的变化。【结果】干旱胁迫下燕麦生长受到显著抑制，喷施HA后燕麦株高和单株叶面积均显著增加；叶片Pn、Gs和Tr显著提高，而Ci显著降低；IAA、GA和ZR显著增加，而ABA显著下降；上述指标均以重度干旱胁迫时喷施HA效果最优。Pn、Tr分别与IAA、GA和ZR极显著正相关，与ABA极显著负相关；Ci与IAA极显著负相关；Gs与ZR和GA极显著正相关，与IAA显著正相关，与ABA极显著负相关。【结论】干旱胁迫条件下喷施腐植酸可以通过调控叶片光合参数和内源激素含量，延缓燕麦衰老，促进其生长发育。 |
| 关键词: 干旱胁迫 腐植酸 燕麦 叶片光合参数 内源激素 |
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| 基金项目:国家重点研发计划项目（2018YFE0107900）；国家自然科学基金项目（32160508） |
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| Effects of humic acid on photosynthesis and endogenous hormones of oat under drought stress |
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ZHU Shanshan,MI Junzhen,ZHAO Baoping,et al
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| Abstract: |
| 【Objective】This study investigated the effects of humic acid (HA) on photosynthetic parameters and endogenous hormones of oats leaves under drought stress,so as to provide guidance for drought resistant cultivation of oats.【Method】The oat variety of “Yanke 2” was selected for pot experiments.HA was sprayed under three water conditions of normal water supply (75% of field water capacity),moderate drought stress (45% of field water capacity) and severe drought stress (30% of field water capacity).The plant height,leaf area per plant and leaf photosynthetic parameters (photosynthetic rate (Pn),stomatal conductance (Gs),transpiration rate (Tr) and intercellular CO2 concentration (Ci)) and endogenous hormones (auxin (IAA),gibberellin (GA),zeatin nucleoside (ZR) and abscisic acid (ABA)) of oats after spraying HA under drought stress were studied.【Result】Oats growth was significantly inhibited under drought stress.After spraying HA,plant height and leaf area per plant of oats increased significantly.Pn,Gs and Tr in leaves increased significantly,while Ci decreased significantly.IAA,GA and ZR increased significantly,while ABA decreased significantly.Among above indexes,the effect of spraying HA under severe drought stress was the best.Pn and Tr had very significantly positive correlation with IAA,GA and ZR,and very significantly negative correlation with ABA.Ci had very significantly negative correlation with IAA.Gs had very significantly positive correlation with ZR and GA,significantly positive correlation with IAA and very significantly negative correlation with ABA.【Conclusion】Spraying HA under drought stress can delay oat senescence and promote oat growth and development by regulating photosynthetic parameters and endogenous hormone contents of oats leaves. |
| Key words: drought stress humic acid oat leaf photosynthetic parameters endogenous hormones |
