| 摘要: |
| 【目的】在全基因组水平对小麦(Triticum aestivum L.)OSCA家族成员（TaOSCAs）进行鉴定，以明确TaOSCA在小麦中的潜在生物学功能。【方法】利用BLASTP结合保守结构域筛选的方法鉴定小麦TaOSCA蛋白。用生物信息学方法分析TaOSCA基因家族成员系统发育关系，基因结构及跨膜结构域；利用公开的转录组数据分析TaOSCA在小麦不同发育阶段不同器官/组织的表达谱；以小麦品种中国春为材料，进行PEG-6000模拟的渗透胁迫处理，用实时荧光定量PCR检测叶片TaOSCAs在渗透胁迫下表达模式。【结果】从小麦中鉴定到35个TaOSCA蛋白；TaOSCAs分为TaOSCAⅠ、TaOSCAⅡ、TaOSCAⅢ和TaOSCAⅣ 4个亚家族，分别有15，16，3和1个成员。基因结构分析结果表明，除TaOSCA4.1-4B外，其余TaOSCA均包含多个外显子。跨膜结构域分析结果表明，除TaOSCA2.3-4B有5个跨膜结构域（TMs）外，其余TaOSCA成员均包含8～11个TMs。TaOSCAs在小麦不同发育时期各器官中的表达结果表明，TaOSCA1.1-3B/3D、TaOSCA1.2-1A/1B/1D、TaOSCA1.3-1A、TaOSCA1.6-1A/1D和TaOSCA3.1-2B/2D在全生育期高水平表达，TaOSCA1.4-2A/2B/2D在不同发育阶段的根组织中均高表达，TaOSCA1.5-1B/1D在生殖生长期穗中高表达，TaOSCA1.6-1B、TaOSCA2.6-3B/3D在生殖生长期籽粒中高表达，表明这些基因可能在根、穗及籽粒发育过程中发挥重要功能。渗透胁迫处理下，TaOSCA2.4和TaOSCA4.1表达下调，TaOSCA2.5的表达无显著变化，TaOSCA1.1、TaOSCA1.2等11个TaOSCA基因表达上调，说明这11个基因可能在小麦苗期响应渗透胁迫中发挥作用。【结论】TaOSCA基因可能在小麦响应渗透胁迫的过程中发挥重要作用。 |
| 关键词: 小麦 干旱胁迫 TaOSCA 系统发育 特异性表达 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金面上项目（32072002） |
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| Genome-wide identification and expression analysis of the OSCA gene family in wheat |
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ZHANG Hongjuan,ZHU Dehe,DU Linying,et al
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| Abstract: |
| 【Objective】Genome-wide identification of OSCA family members (TaOSCAs) was conducted to clarify the potential biological functions of TaOSCA in wheat.【Method】The TaOSCA members in wheat were identified by BLASTP combined with the screening of conserved domains.Phylogenetic,gene structure and transmembrane domain of TaOSCA gene family members were analyzed by bioinformatics methods.The expression profiles of TaOSCA in different organs/tissues of wheat at different developmental stages were analyzed using published transcriptome data.PEG-simulated osmotic stress treatment was performed using Chinese spring wheats,and the expression patterns of TaOSCAsin leaves under osmotic stress were detected by real-time PCR (RT-qPCR).【Result】A total of 35 TaOSCAs were identified in wheat,and members of the TaOSCA family were divided into 4 subfamilies of TaOSCAⅠ,TaOSCAⅡ,TaOSCAⅢ and TaOSCAⅣ,with 15,16,3 and 1 members,respectively.The gene structure analysis showed that TaOSCA genes contained multiple exons except TaOSCA4.1-4B.The transmembrane domain analysis showed that TaOSCA proteins contained 8-11 transmembrane domains (TMs) except for TaOSCA2.3-4B with 5 TMs.The expression profiles of TaOSCAs in different stages and tissues of wheat showed that TaOSCA1.1-3B/3D,TaOSCA1.2-1A/1B/1D,TaOSCA1.3-1A,TaOSCA1.6-1A/1D and TaOSCA3.1-2B/2D were highly expressed in the whole growth stage,TaOSCA1.4-2A/2B/2D were highly expressed in the root tissue in different stages,TaOSCA1.5-1B/1D were highly expressed in the panicle during the reproductive growth stage,and TaOSCA1.6-1B and TaOSCA2.6-3B/3D were highly expressed in grains during reproductive growth.These genes may play important functions in root,spike and grain development.The RT-qPCR detection of leaves under osmotic stress treatment showed that TaOSCA2.4 and TaOSCA4.1 expressions were inhibited under osmotic stress,TaOSCA2.5 expression was insignificantly changed,while expressions of 11 TaOSCAs including TaOSCA1.1 and TaOSCA1.2 were up-regulated under osmotic stress.It is indicated that these genes may play a role in response of wheat seedlings to osmotic stress.【Conclusion】TaOSCA gene may play an important role in responses of wheat to osmotic stress. |
| Key words: wheat drought stress TaOSCA phylogenetic analysis specific expression |
