| 摘要: |
| 【目的】明确福建省水稻稻瘟病菌对吡唑醚菌酯、戊唑醇、丙环唑和多菌灵的敏感性，为稻瘟病菌的抗药性监测及防治提供依据。【方法】以从福建将乐、沙县、宁化、建阳、南靖、上杭6个地区采集分离的116株稻瘟病菌为试验材料，采用菌丝生长速率法测定吡唑醚菌酯、戊唑醇、丙环唑和多菌灵对供试菌株的有效抑制中浓度(EC50)，分析供试菌株对吡唑醚菌酯、戊唑醇、丙环唑和多菌灵的敏感性及其相关性，建立供试菌株对4种杀菌剂的敏感性频率分布图，确定其敏感基线。【结果】吡唑醚菌酯、戊唑醇、丙环唑和多菌灵对供试稻瘟病菌菌株的EC50分别为0.001 2～0.012 8，0.209 8～0.632 3，0.196 8～2.273 0和0.217 6～0.616 1 μg/mL，EC50平均值分别为0.004 7，0.361 9，0.812 4和0.427 9 μg/mL，表明福建省稻瘟病菌对吡唑醚菌酯最敏感，其次为戊唑醇、多菌灵和丙环唑。不同地区稻瘟病菌菌株对同一种杀菌剂的敏感性存在差异，同一地区稻瘟病菌菌株对同一种杀菌剂的敏感性也存在差异；敏感性频率分布的正态性检验结果显示，福建省稻瘟病菌对吡唑醚菌酯、戊唑醇、丙环唑和多菌灵的敏感性频率分布均为连续性单峰曲线，且未出现敏感性下降的亚群体，因此，可分别将吡唑醚菌酯、戊唑醇、丙环唑和多菌灵对供试菌株的EC50平均值（0.004 7，0.361 9，0.812 4和0.427 9 μg/mL）作为其敏感基线，用于田间菌株的抗药性监测。供试稻瘟病菌对吡唑醚菌酯的敏感性与戊唑醇、丙环唑、多菌灵的敏感性之间无显著相关性。【结论】4种杀菌剂对福建稻瘟病菌的敏感性依次为吡唑醚菌酯＞戊唑醇＞多菌灵＞丙环唑；吡唑醚菌酯可作为防治稻瘟病的主要杀菌剂，并与戊唑醇、多菌灵和丙环唑轮换或混合使用，避免或延缓稻瘟病菌对其产生抗药性。 |
| 关键词: 稻瘟病菌 杀菌剂 吡唑醚菌酯 敏感基线 |
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| 基金项目:福建省属公益类科研院所专项（2019R1024-6）；福建省农业科学院创新平台专项（CXPT0022019005）；福建省农业科学院对外合作项目（DEC201908）;国家重点研发计划项目(2016YFD0300707)；福建农业科学院科技创新团队项目(CXTD2021016);福建农业科学院5511项目(KXXYJBG0041) |
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| Sensitivity of Magnaporthe oryzae in Fujian to 4 fungicides |
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RUAN Hongchun,SHI Niuniu,TIAN Peiyu,et al
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| Abstract: |
| 【Objective】This study determine the susceptibility of rice blast pathogen Magnaporthe oryzae to pyraclostrobin,tebuconazole,propiconazole and carbendazim in Fujian to provide basis for monitoring and controlling of resistance to M.oryzae.【Method】A total of 116 M.oryzae strains collected and isolated from Jiangle,Shaxian,Ninghua,Jianyang,Nanjing and Shanghang in Fujian were used as tested materials.The mycelial growth rate method was used to detect half maximal effective concentration (EC50) of pyraclostrobin,tebuconazole,propiconazole and carbendazim on tested strains.The sensitivity and correlation between tested strains and the four fungicides were also conducted to establish sensitivity frequency distribution and determined sensitivity baselines.【Result】The EC50 values of pyraclostrobin,tebuconazole,propiconazole and carbendazim against tested M.orygae strains were 0.001 2―0.012 8,0.209 8―0.632 3,0.196 8―2.273 0 and 0.217 6―0.616 1 μg/mL with averages of 0.004 7,0.361 9,0.812 4 and 0.427 9 μg/mL,respectively.It indicated that M.orygae in Fujian was the most sensitive to pyraclostrobin,followed by tebuconazole,carbendazim and propiconazole.There were differences in susceptibility of M.orygae strains to same fungicides in different areas,and there were also differences in susceptibility of M.orygae strains to same fungicides in same areas.The normality test of susceptibility frequency distribution showed that M.orygae strains in Fujian had continuous single peak curves against pyraclostrobin,tebuconazole,propiconazole and carbendazim,without subgroups with reduced sensitivity.Therefore,the average EC50 values (0.004 7,0.3619,0.812 4 and 0.427 9 μg/mL) of pyraclostrobin,tebuconazole,propiconazole and carbendazim against tested strains could be used as their sensitive baselines for monitoring the resistance of strains in the field.There was no significant correlation between the susceptibility of tested M.oryzae strains to pyraclostrobin and the susceptibility to other three fungicides.【Conclusion】The sensibility of the four fungicides tested on hyphae growth of M.oryzae strains was in the order of pyraclostrobin>tebuconazole>carbendazim>propiconazole.As the main fungicide,pyraclostrobin could be used in rotation or mixture with tebuconazole,carbendazim and propiconazole to avoid or delay the development of resistance in M.oryzae. |
| Key words: Magnaporthe oryzae fungicides pyraclostrobin sensitivity baseline |
