| 摘要: |
| 【目的】比较分析干旱耐受型（Han21）和敏感型（B73）玉米自交系响应干旱胁迫的转录组数据，为理解玉米应答干旱胁迫的分子机制提供依据。【方法】利用高通量测序技术获得Han21、B73在正常和干旱条件下的转录组，随后利用一系列生物信息学方法进行转录组数据质控（FastQC）、测序数据与基因组的序列比对（HISAT2）、基因表达定量分析（StringTie）、差异表达分析（edgeR）、转录本转换事件分析（deepTS）以及基因功能富集分析（topGO）。【结果】B73和Han21玉米自交系在正常和干旱条件下的差异表达基因分别有917和2 832条，其中转录因子分别有80和214条；干旱胁迫下，双因素分析发现1 475条基因在B73和Han21中的表达存在显著差异，这些基因参与细胞壁合成、碳水化合物及多糖代谢等生物学过程；1 475条差异表达基因中，B73和Han21分别有28和36条基因发生了TS事件，其中15条基因在B73和Han21中的转录本转换事件呈现出相反的转换模式。【结论】基因和转录本层面的比较转录组研究，有助于发现玉米响应和抵御干旱胁迫的重要候选基因。 |
| 关键词: 玉米 干旱胁迫 差异表达 转录本转换 生物信息学 |
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| 基金项目:陕西省青年科技新星项目（2017KJXX-67）；中央高校基本科研业务费资助项目（2452020041） |
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| Comparative transcriptome analysis of drought stress responses in drought-tolerant and drought-sensitive maize inbred lines |
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LIU Chunni,SONG Minggui,CHEN Siyuan,et al
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| Abstract: |
| 【Objective】Comparative transcriptome analysis of drought tolerant (Han21) and drought sensitive (B73) maize inbred lines under well watered and drought stressed conditions was conducted for understanding drought stress response mechanisms of maize.【Method】The drought stress responsive transcriptomes of Han21 and B73 were obtained using high-throughput sequencing technologies.A series of bioinformatics analysis,including quality control of sequencing reads (FastQC),read genome alignments (HISAT2),estimation of gene expression abundance (StringTie),differential expression analysis (edgeR),transcriptional switch analysis (deepTS) and gene ontology (GO) enrichment analysis (topGO),were performed.【Result】A total of 917 (80 transcription factors) and 2 832 (214 transcription factors) genes were differentially expressed in B73 and Han21 inbred lines in response to drought stress,respectively.Two way differential expression analysis identified a total of 1 475 genes with significant differences in expression changes between B73 and Han21 under drought stress.These differentially expressed genes were significantly enriched in several biological processes including cell wall biogenesis and carbohydrate metabolic processes.Among 1 475 differentially expressed genes,transcriptional switch events occurred in B73 and Han21 inbred lines corresponding to 28 and 36 genes,respectively.Additionally,15 differentially expressed genes had transcriptional switch events with different switch patterns.【Conclusion】Comparative transcriptome analysis at gene and transcription levels would be valuable for the identification of candidate functional genes involved in drought stress response and tolerance in maize. |
| Key words: maize drought stress differential expression transcriptional switch bioinformatics |
