| 摘要: |
| 【目的】研究半高丛越橘的耐盐性和耐盐范围，为其在盐碱地的引种栽培提供参考。【方法】以2年生半高丛越橘品种‘北陆’为材料，研究不同NaCl处理(0（CK），30，60，90，120，150，180和210 mmol/L)对越橘叶片形态、生理生化、叶绿素含量、光合作用参数及荧光参数指标的影响，筛选出越橘的耐盐范围，评估其耐盐性。【结果】(1)越橘植株在210 mmol/L NaCl处理下38 d失绿；45 d叶片干枯卷曲，枝条开始失绿；62 d枝条已基本失绿，植株接近死亡；且解除胁迫换土栽培后，仍无生命迹象。越橘叶片的叶面积、叶长、叶宽、鲜质量和干质量均在90 mmol/L NaCl处理下达到最大；相对含水量以60 mmol/L NaCl处理最高，且较CK显著增加；比叶重在120 mmol/L NaCl处理下出现最大值，且与CK差异极显著（P＜0.01）。(2)随着NaCl浓度增大，越橘叶片可溶性蛋白含量明显增加，CAT活性上升，POD活性表现为先升高后下降，且高浓度（≥150 mmol/L）NaCl处理越橘叶片Chl a、Chl b和Chl含量降低。(3)NaCl浓度≤90 mmol/L时，越橘叶片ΦPSⅡ、qp、NPQ和ETR均高于CK；NaCl浓度＞90 mmol/L时，各项指标均呈下降趋势，且180 mmol/L NaCl处理Fv/Fm、Pn、Tr和Gs均下降，Ci则升高。【结论】越橘品种‘北陆’有一定的耐盐能力，其耐盐浓度为0～90 mmol/L，210 mmol/L NaCl是该品种耐盐最大临界值。 |
| 关键词: 半高丛越橘 耐盐性 叶片形态 光合生理 叶绿素荧光 |
| DOI： |
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| 基金项目:吉林省教育科学“十三五”规划项目(GH170250)；淄博市科学技术发展计划项目（2017kj080008）；淄博市校城融合发展计划项目（2018ZBXC040） |
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| Impacts of NaCl treatment on leaf morphology and photosynthetic physiological characteristics of semi-high-bush blueberry seedlings |
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ZHANG Xiaoting,WEI Jianchen,XU Zhenbiao,et al
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| Abstract: |
| 【Objective】The salt stress and salt stress range of semi-high-bush blueberry were studied to provide reference for the introduction and cultivation in saline-alkali land. 【Method】The 2-year-old Vaccinium L.‘Northland’ seedlings were selected to study the effect of different NaCl concentrations (0(CK),30,60,90,120,150,180 and 210 mmol/L) on morphology,physiological and biochemical indexes,chlorophyll content, photosynthetic physiology and chlorophyll fluorescence of blueberry leaves.The range of salt tolerance was also screened to evaluate its salt tolerance.【Result】(1) The blueberry plants lost green in 38 days,the leaves were dry and curled and the branches began to lose green in 45 days, and the branches basically lost green and plants were nearly dead in 62 days in the 210 mmol/L NaCl treatment.The plants did not come back to life after the stress was released.The leaf area,length,diameter,fresh weight and dry weight of blueberry leaves reached the maximum when the NaCl concentration was 90 mmol/L.The relative water content was the highest when the NaCl concentration was 60 mmol/L,and it was significantly higher than the control.The specific leaf weight reached the maximum when the NaCl concentration was 120 mmol/L,and the difference from control was extremely significant (P<0.01).(2) The soluble protein content and CAT of blueberry leaves were increased significantly when the NaCl concentration increased, and the activity of POD was increased firstly before decreasing.When the NaCl concentration was higher than ≥150 mmol/L,the Chl a,Chl b and Chl in leaves were significantly reduced.(3) The ΦPSII,qp,NPQ and ETR of leaves increased in comparison with the control when the NaCl concentration was below 90 mmol/L.They decreased when the NaCl concentration was greater than 90 mmol/L.When the NaCl concentration was 180 mmol/L,Fv/Fm,Pn,Tr and Gs decreased,but Ci increased.【Conclusion】The Vaccinium L.‘Northland’ seedlings had certain ability to adapt NaCl stress,and the salt tolerance range was 0-90 mmol/L NaCl with the maximum of 210 mmol/L NaCL. |
| Key words: semi-high-bush blueberry salt tolerance leaf morphology photosynthetic physiology chlorophyll fluorescence |
