| 摘要: |
| 【目的】阐明臭氧处理对陈番茄和黄瓜种子萌发及种子活力的影响。【方法】以放置1年的陈番茄和黄瓜种子作为研究对象,用80,120和160 mg/m3的臭氧分别处理番茄和黄瓜种子30 min,研究不同臭氧处理对番茄和黄瓜种子表面微生物菌落数以及种子发芽势、发芽率和活力(SOD、POD活性和MDA含量)的影响,并对各生理指标的相关性进行分析。【结果】用160 mg/m3的臭氧处理30 min,番茄和黄瓜种子表面微生物的抑制率均达到最大值,分别为97.4%和99.5%。用80 mg/m3的臭氧处理30 min,番茄种子的发芽势、发芽率、SOD活性和POD活性均达到最大值,分别为68.0%,94.5%,193.8 U/g和2 193.4 U/g,MDA含量达到最小值,为19.4 nmol/g。用160 mg/m3的臭氧处理30 min,黄瓜种子的发芽势、发芽率、SOD活性和POD活性均达到最大值,分别为96.5%,99.5%,160.3 U/g和2 300.4 U/g,MDA含量达到最小值,为10.8 nmol/g。臭氧处理后,番茄和黄瓜种子SOD和POD活性与发芽势和发芽率呈显著正相关,而MDA含量与发芽势和发芽率呈显著负相关。【结论】适宜浓度的臭氧处理后可以抑制种子表面微生物的生长,提高种子的发芽率和活力。 |
| 关键词: 种子萌发 臭氧处理 番茄 黄瓜 种子活力 |
| DOI: |
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| 基金项目:山西省重点研发计划项目“保护地非农药(臭氧)病虫害绿色防控设备及技术的研究”(201703D22111288) |
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| Effect of ozone treatment on germination of tomato and cucumber seeds |
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ZHANG Zhijia,,LIU Hongyan,ZHAI Haixiang,et al
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| Abstract: |
| 【Objective】The effects of ozone treatment on aged seed germination and seed vigor of tomato and cucumber were analyzed to provide theoretical basis for agricultural production practice.【Method】The effects of different ozone treatments of 80,120 and 160 mg/m3 on surface microbial colony numbers,germination potential,germination rate and seed vigor(SOD,POD and MDA)of tomato and cucumber seeds were studied and the correlations of indexes were analyzed.【Result】The inhibition rates of surface microorganisms reached maximum values of 97.4% and 99.5% when tomato and cucumber seeds were treated by 160 mg/m3 ozone for 30 minutes.When tomato seeds were treated by 80 mg/m3 ozone for 30 minutes,the germination potential,germination rate and activities of SOD and POD also reached maximum values of 68.0%,94.5%,193.8 U/g and 2 193.4 U/g respectively.However,MDA content reached the minimum value of 19.4 nmol/g.When cucumber seeds were treated by 160 mg/m3 ozone for 30 minutes,the germination potential,germination rate and activities of SOD and POD reached maximum values of 96.5%,99.5%,160.3 U/g and 2 300.4 U/g,while MDA content reached the minimum value of 10.8 nmol/g.The activities of SOD and POD had positive correlation with germination potential and germination rate,while MDA had negative correlation with germination potential and germination rate after treatment.【Conclusion】 Proper ozone treatment can inhibit the growth of microorganisms on seed surface and improve the germination rate and seed vigor. |
| Key words: seed germination ozone treatment tomato cucumber seed vigor |