| 摘要: |
| 【目的】研究饲粮n-6/n-3多不饱和脂肪酸( PUFA) 配比对育成生长期雄性北极狐和银狐血液生化指标的影响，为其饲粮的配制提供参考。【方法】采用双因素随机设计，以品种(北极狐和银狐)和n-6/n-3 PUFA比例(3,6,9和18)为试验因素,选取102日龄健康雄性北极狐和银狐各48只，预饲7 d后均随机分成4组，每组12个重复，每重复1只狐，分别饲喂不同n-6/n-3 PUFA 配比的试验饲粮46 d，试验结束后采集血清样品，测定试狐血清葡萄糖(GLU)、甘油三酯(TG)、胆固醇(CHO)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、免疫球蛋白IgG和IgM、白介素-2(IL-2)、总蛋白(TP)、白蛋白(ALB)含量，并计算球蛋白(GLOB)质量浓度和白蛋白/球蛋白(A/G)值。【结果】1)北极狐血清TG、CHO、HDL-C浓度极显著高于银狐(P＜0.01)，银狐血清LDL-C浓度显著高于北极狐(P＜0.05)；n-6/n-3 PUFA比例单一作用对血清CHO、LDL-C和GLU浓度有显著影响，对TG、HDL-C浓度无显著影响；品种和n-6/n-3 PUFA比例交互作用对GLU浓度有显著影响，对LDL-C浓度有极显著影响，对TG, CHO、HDL-C浓度无显著影响。2)北极狐血清IL-2质量浓度极显著高于银狐(P＜0.01)；n-6/n-3 PUFA比例单一作用对血清IL-2质量浓度有显著影响，对IgM和IgG质量浓度无显著影响；品种和n-6/n-3 PUFA比例交互作用对IL-2、IgM质量浓度有显著影响，对IgG质量浓度无显著影响。3)银狐血清TP、ALB、GLOB质量浓度显著或极显著高于北极狐；n-6/n-3 PUFA比例单一作用及与品种交互作用对血清TP、ALB、GLOB、A/G质量浓度无显著影响。【结论】北极狐和银狐生理代谢存在差异，北极狐血清脂类指标(除血清LDL-C外)含量均高于银狐，银狐血清蛋白类指标均高于北极狐，n-6/n-3 PUFA比例为18的饲粮可降低北极狐和银狐血清糖脂指标，维持机体糖脂平衡；n-6/n-3 PUFA比例为3的饲粮可使北极狐和银狐机体处于较好的免疫状态。 |
| 关键词: 饲料配比 n-6/n-3 PUFA；北极狐；银狐；血清生化指标 |
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| 基金项目:中国农业科学院科技创新工程项目（CAAS-ASTIP-2018-ISAPS);吉林省重点科技成果转化项目(20160307022NY) |
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| Effects of dietary n-6/n-3 PUFA ratio on serum biochemical parameters in male arctic foxes and silver foxes during growing period |
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ZHONG Wei,ZHANG Ting,LUO Jing,et al
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| Abstract: |
| 【Objective】This experiment was conducted to study the effect of dietary n-6/n-3 PUFA ratio on serum biochemical parameters of male arctic foxes and silver foxes during growing period.【Method】The double factor design was used with one factor of breed including arctic fox and silver fox and the other factor of n-6/n-3 PUFA ratios including 3，6，9 and 18.Forty-eight male arctic foxes and silver foxes at the age of 102 days were randomly divided into 4 groups after 7 days adaption,with 12 replicates per group and 1 fox per replicate.The foxes were fed on experimental diets with different n-6/n-3 PUFA ratios for 46 days.After the experiment,serum samples were collected for the determination of glucose (GLU),triglyceride (TG),cholesterol(CHO),low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C),immunoglobulin G (IgG) and immunoglobulin M (IgM),interleukin 2 (IL-2),total protein (TP),albumin (ALB) contents.Both globulin (GLOB) content and albumin/globulin (A/G) ratio were calculated.【Result】1) Serum contents of TG,CHO and HDL-C of arctic fox were extremely significantly higher than that in silver fox (P＜0.01),while LDL-C content of silver fox was significantly higher than that in arctic fox (P＜0.05).The n-6/n-3 PUFA ratio had significant effect on serum CHO,LDL-C and GLU contents, while had no significant effect on TG and HDL-C contents.Interaction of n-6/n-3 PUFA ratio and breed significantly affected serum GLU content and extremely significantly affected serum LDL C content,while did not significantly affect serum TG,CHO and HDL-C contents.2) Serum IL-2 content in arctic fox was significantly higher than that in silver fox (P＜0.01).The n-6/n-3 PUFA ratio significantly affected serum IL-2 content,while did not significantly affect serum IgM and IgG contents.Interaction of n-6/n-3 PUFA ratio and breed significantly affected serum IL-2 and IgM contents,while did not significantly affect serum IgG content.3) Serum TP,ALB and GLOB contents in silver fox were significantly or extremely significantly higher than that in arctic fox.The n-6/n-3 PUFA ratio and interaction of n-6/n-3 PUFA ratio and breed did not significantly influence serum TP,ALB,GLOB and A/G contents.【Conclusion】 There were differences in physiological metabolism between arctic foxes and silver foxes. Except for LDL-C,serum lipid indexes of arctic foxes were higher than that of silver foxes,while serum protein parameters of silver foxes were higher than that of arctic foxes.When the dietary ratio of n-6/n-3 PUFA ratio was 18,serum glycolipid parameters of foxes were reduced and glycolipid balance in body was maintained.When the n-6/n-3 PUFA ratio was 3,foxes were in good immune status. |
| Key words: feed proportion n-6/n-3 PUFA arctic fox silver fox serum biochemical parameters |
