| 摘要: |
| 【目的】优化乙醇/(NH4)2SO4双水相体系提取藏药瑞香狼毒多糖的工艺参数，并分析瑞香狼毒多糖的抑菌及抗氧化活性，为瑞香狼毒功能产品的研发及多元化应用提供理论依据。【方法】以不同的料(g)液(mL)比（1∶30，1∶40，1∶50）、提取温度（45，55，65 ℃）和提取时间（20，30，40 min）作为考察因素，以多糖的提取率作为评价指标，采用响应曲面优化分析确定瑞香狼毒多糖最佳提取工艺参数。利用硫酸亚铁 水杨酸氧化法和邻苯三酚自氧化法分别评估瑞香狼毒多糖（质量浓度分别为0.6，1.2，1.8，2.4，3.0，3.6 mg/mL）对羟基自由基（·OH）和过氧负离子自由基（·O2-2）的体外清除能力。采用滤纸片扩散法研究瑞香狼毒多糖（质量浓度为0.3和3.0 mg/mL）的抑菌活性。【结果】料(g)液(mL)比1∶40、提取温度55 ℃、提取时间30 min为最优的工艺参数，在该条件下瑞香狼毒多糖的提取率最高，达15.34%。3.0 mg/mL瑞香狼毒多糖对·OH的清除率最大，为29.4%；2.4 mg/mL瑞香狼毒多糖对·O2-2的清除率最大，为24.7%。抑菌试验结果表明，3.0 mg/mL瑞香狼毒多糖对金黄色葡萄球菌和大肠杆菌高度敏感，对枯草芽孢杆菌中度敏感。【结论】确定了瑞香狼毒多糖乙醇/(NH4)2SO4双水相体系提取的最佳工艺参数，建立了因素与提取率之间的二次函数模型；瑞香狼毒多糖具有一定的体外抑菌、抗氧化活性。 |
| 关键词: 瑞香狼毒 多糖 提取抑菌活性 抗氧化活性 |
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| 基金项目:甘肃省高等学校科研项目 (2016A-093) |
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| Optimization of polysaccharides extraction from Tibetan medicine Stellera chamaejasme and the antibacterial and antioxidant activities |
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QI Xiaoni,MA Mei,LI Zhenliang,et al
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| Abstract: |
| 【Objective】In this work, the extraction parameters of Stellera chamaejasme polysaccharides (SCP) using the aqueous two phase system of ethanol/ammonium sulphate were optimized and the antibacterial and antioxidant activities of SCP were evaluated to provide theoretical basis for research,development and diversification of the products.【Method】The optimal extraction parameters were determined using response surface method for extraction temperature (45,55 and 65 ℃),extraction time (20,30 and 40 min) and ratio of liquid to materials(1∶30,1∶40 and 1∶50) with SCP yield as index.The scavenging effect of hydroxyl radical and superoxide radical were detected with the oxidation of ferrous sulfate-salicylic acid and self oxidation of pyrogallic acid system under the mass concentrations of 0.6，1.2,1.8，2.4，3.0 and 3.6 mg/mL.The antibacterial activities at mass concentrations of 0.3 and 3.0 mg/mL were determined by filter paper diffusion method.【Result】The optimum extraction conditions were extraction temperature 55 ℃,extraction time 30 min and ratio of liquid to materials 1∶40 (g∶mL) with the highest SCP yield of 15.34%.SCP exhibited significant protection against hydroxyl radical and superoxide ion induced oxidative damage and the maximum scavenging activities were 29.4% (3.0 mg/mL) and 24.7%(2.4 mg/mL),respectively.The 3.0 mg/mL SCP presented high sensitivity to Colibacillus and Staphylococcus aureus,but medium sensitivity to Bacillus subtilis. 【Conclusion】 The optimum SCP extraction parameters by the aqueous two phase system of ethanol/ammonium sulphate were determined and a quadratic multinomial regression model between extraction factors and yield was established.SCP showed antioxidant and antimicrobial activities in vitro. |
| Key words: Stellera chamaejasme polysaccharides extraction antioxidant activity antibacterial activity |
