| 摘要: |
| 【目的】 研究胶质芽孢杆菌（Bacillus mucilaginous）MY6-2的絮凝特性及其对重金属Pb2+的吸附作用，为微生物絮凝剂的实际应用提供参考。【方法】通过发酵、醇沉、透析、冷冻干燥得到胶质芽孢杆菌MY6-2生物絮凝剂；研究pH、絮凝剂用量、金属助凝剂及其用量、加热对MY6-2絮凝剂活性的影响；分析溶液pH、Pb2+初始质量浓度及絮凝剂用量对MY6-2絮凝剂吸附Pb2+的影响。【结果】MY6-2絮凝剂的主要成分为多糖，占样品的65.68%;最佳絮凝条件为：pH＞7.0,絮凝剂用量20 mL/L，助凝剂金属离子为Ca2+（质量分数1%，用量为20 mL/L），在此条件下MY6-2絮凝活性可达到95%以上。在100 ℃水浴中加热30 min絮凝活性无明显变化，表明该絮凝剂具有良好的热稳定性。MY6-2絮凝剂对Pb2+的最佳吸附条件为:pH 4.0、Pb2+初始质量浓度100～500 mg/L、絮凝剂用量80 mL/L，在此条件下,MY6-2絮凝剂对Pb2+的絮凝活性均在80%以上。【结论】MY6-2絮凝剂具有较高的絮凝活性，对重金属Pb2+具有较高的吸附活性，且生产成本低，安全性高，具有较高的推广应用价值。 |
| 关键词: 胶质芽孢杆菌 生物絮凝剂 絮凝活性 Pb2+ 生物吸附 |
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| 基金项目:国家自然科学基金项目(31270665)；陕西省社会发展科技攻关项目（2015SF263）；西安市科技计划项目（CX1259（3））；陕西省矿产资源勘查与综合利用重点实验室项目（2015-001） |
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| Characteristics of Bacillus mucilaginosus microbial flocculant and its Pb2+ adsorption application |
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LAN Meng,LIANG Yuyan,GAO Xiaofeng,et al
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| Abstract: |
| 【Objective】This study explored the characteristics of a microbial flocculant produced by Bacillus mucilaginosus MY6-2 and its adsorption of Pb2+ to provide basis for actual applications.【Method】A novel bio-flocculant was obtained from Bacillus mucilaginosus MY6-2 by alcohol precipitation,dialysis and lyophilization.The effects of pH,MBF dosage,cation supplement and temperature on flocculating activity were studied.The adsorption of Pb2+ was tested using batch method with respect to pH,initial metal ion concentration and MBF dosage.【Result】The main component of MBF MY6-2 was extracellular polysaccharide.Under the optimum condition of pH＞7.0,MBF dosage 20 mL/L Kaolin suspension,and 1% CaCl2 20 mL/L,the obtained flocculating activity was >95%.The flocculating activity remained after being heated to 100 ℃ for 30 min,indicating that MBF MY6-2 had high thermal stability.The optimum adsorption conditions were pH 4.0,initial ion concentration 100-500 mg/L and MBF dosage 80 mL/L.The adsorption rate was maintained above 80% under these conditions.【Conclusion】MBF MY6-2 is a cost-effective and safe microbial flocculant, and it has high flocculating activity and adsorption activity of Pb2+. |
| Key words: Bacillus mucilaginous microbial flocculant flocculating activity Pb2+ biosorption |
