| 摘要: |
| 【目的】从秋海棠中筛选植物促生菌，并探究其对植物促生防病作用机制。【方法】将健康的秋海棠植株整株挖出，采集健康部位的根部和茎部，清洗后进行表面消毒，并切割为6~8段（每段长度约0.5 cm），将切段的材料分别置于3种筛选培养基（PDA培养基、YMA培养基、牛肉膏蛋白胨培养基）中进行培养，以筛选出目标促生菌，并分析促生菌的促生能力以及抑菌活性；最后采用盆栽试验和大田试验，均以无菌水处理为对照，分析了促生菌对大豆和番茄生长的影响。【结果】从秋海棠根部和茎部分离获得22株菌株，其中11株菌具有多种促生和防病作用。盆栽试验结果表明，与对照相比，单一促生菌处理大豆的株高、根长、茎长、茎直径和果实鲜质量分别平均增加27.33%，15.84%，23.46%，13.20%，22.39%；Y-01与Y-02混合菌液处理的促生效应明显优于单一促生菌，大豆株高、根长、茎长、茎直径和果实鲜质量较对照分别增加了37.42%，25.65%，38.53%，16.42%和34.84%。大田试验结果表明，灌根处理15和60 d后，11株单一促生菌和Y-01与Y-02混合菌液处理能明显促进番茄的生长。【结论】从秋海棠中筛选到了多株促生菌菌株，其对植物生长有明显的促进作用。 |
| 关键词: 秋海棠 植物促生菌 促生效应 大田试验 |
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| 基金项目:陕西省科技统筹创新工程项目(2012CGX7,2016TTC-N-1);延安市科技局重大专项(2014CGZH-06);陕西省教育厅服务地方专项计划项目(16JF029);延安市菌草工程研究中心专项基金项目;延安市生物资源开发与利用科技创新团队专项基金项目(2016CXTD-01)；延安大学产学研合作研发项目;延安大学“陕北微生物资源与利用研究中心”科研机构专项 |
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| Screening of plant growth promotion bacteria from Begonia grandis and investigation of their promoting effects |
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DENG Zhenshan,DUAN Yangyang
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| Abstract: |
| 【Objective】Plant growth promoting bacteria (PGPB) was isolated from Begonia grandis,and the mechanism of growth promoting was studied.【Method】Whole plants of Begonia grandis were collected and roots and stems of health plants were collected and cut to 6－8 segments (0.5 cm each) after cleaning and surface disinfection.The segments were cultured in PDA,YMA,and NA media for screening strains with plant growth promotion and inhibitory effects against pathogen.Furthermore,soybean and tomato growth enhancement and antagonism were tested by plant inoculation studies with these isolates under soybean and tomato pot experiments.【Result】A total of 22 strains were isolated from stem and root of Begonia grandis,11 of which had multiple effects of growth promotion and disease prevention.Under pot and field experiment conditions,the plant height,root length,stem length,stem diameter,weight,and fruits fresh weight of plants inoculated with single strain were increased by 27.33%,15.84%,23.46%,13.20%,and 22.39% compared with the control.The combination of Y-01 and Y-02 had better growth promotion effects and plant height,root,length,stem length,stem diameter and fruit fresh weight increased by 37.42%,25.65%,38.53%,16.42% and 34.84%,respectively.Under field experiment condition after 15 and 60 d root irrigation,the results showed that the single strain and the combination of Y-01 and Y-02 could clearly accelerate the growth of tomato.【Conclusion】Multiple strains with growth promotion and disease prevention effects were isolated from Begonia grandis. |
| Key words: Begonia grandis plant growth promoting bacteria promoting effects field experiment |
