| 摘要: |
| 【目的】研究LED红蓝不同光质比和光照强度对金娃娃萱草试管苗生长的影响，为提供高品质的金娃娃萱草试管苗提供理论依据。【方法】采用LED光源的红光R（主波长640 nm）、蓝光B（主波长464 nm），设计5种不同光质配比（100% R、80% R+20% B、70% R+30% B、50% R+50% B、100% B），以普通荧光灯作为对照（CK），从中筛选出最佳光质比；在最佳光质比条件下，设置不同光照强度(30，40，50，60 μmol/(m2·s) )，测定不同光质比和光照强度处理下金娃娃萱草试管苗的形态指标、叶绿素、类胡萝卜素、可溶性糖、可溶性蛋白含量以及根系活力。【结果】金娃娃萱草株高和可溶性蛋白含量以单一蓝光处理最大，最大根长和可溶性糖含量以单一红光处理最大，叶数、叶绿素、类胡萝卜素含量和根系活力均以70% R+30% B处理最大，根数和干鲜质量均以80% R+20% B处理最大。株高、叶数、根数、干鲜质量、叶绿素及可溶性蛋白含量均以光照强度为50 μmol/(m2·s)处理最大，最大根长以光照强度为30 μmol/(m2·s)处理最大，可溶性糖含量和根系活力以光照强度为60 μmol/(m2·s)处理最大。【结论】当LED红蓝光质比为70% R+30% B、光照强度为50 μmol/(m2·s)时，更适于金娃娃萱草试管苗的生长及品质的提高。 |
| 关键词: LED 红蓝光 光强 金娃娃萱草 试管苗 |
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| 基金项目:河南省产学研合作项目（162107000068）；国家农业科技成果转化资金项目（2012D0001018） |
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| Effects of light quality ratio and intensity of red/blue light on growth of Hemerocallis middendorfii plantlets in vitro |
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SHANG Wenqian,WANG Zheng,HE Songlin,et al
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| Abstract: |
| 【Objective】The effects of different light quality ratios and intensities of LED red/blue light on growth of Hemerocallis middendorfii plantlets in vitro were studied to provide theoretical evidence for cultivating high quality Hemerocallis middendorfii plantlets in vitro.【Method】 The Hemerocallis middendorfii plantlets in vitro were cultured under five different LED ratios （100% R,80% R+20% B,70% R+30% B,50% R+50% B,and 100% B).Using ordinary fluorescent lamps as control (CK),the best quality ratio was screened.With the best quality ratio,different light intensities (30，40，50，and 60 μmol/(m2·s)) were tested to examine the effects on morphology,chlorophyll content,carotenoid content,soluble sugar content,soluble protein content,and root activity.【Result】 The plant height and soluble protein content reached maximum under monochromatic 100% B while the root length and soluble sugar content reached maximum under monochromatic 100% R.The leaf number,chlorophyll content,carotenoid content and root activity reached maximum in 70% R+30% B treatment.The root number and dry and fresh quality reached maximum in 80% R+20% B.The plant height,leaf number,root number,dry and fresh quality,chlorophyll content and soluble protein content all reached maximum with 50 μmol/(m2·s) intensity,the root length reached maximum under 30 μmol/(m2·s) intensity,and the sugar content and root activity reached maximum under 60 μmol/(m2·s) intensity. 【Conclusion】 The plantlets in vitro grew well under 70% R+30% B and 50 μmol/(m2·s) intensity. |
| Key words: LED red/blue light light intensity Hemerocallis middendorfii plantlets in vitro |
