| 摘要: |
| 【目的】探讨中国荷斯坦牛脂肪酸脱氢酶2（FADS2）基因编码区c.908 C>T突变对泌乳性能和乳中脂肪酸组成的影响。【方法】以江苏某大型奶牛场865头中国荷斯坦牛为材料，用飞行时间质谱法对FADS2基因编码区c.908 C>T突变位点进行检测，并用最小二乘模型分析该位点对泌乳性状和乳中脂肪酸组成的影响。【结果】FADS2基因 c.908 C>T 位点有3种基因型：CC、CT和TT，优势基因型为 CT 型，C基因为优势等位基因。关联分析发现，该位点对日产奶量、体细胞评分（SCS）及乳脂、乳糖、总固体含量5个性状的影响均达到显著水平(P<0.05)，对乳蛋白和乳中尿素氮含量的影响不显著(P>0.05)。多重比较表明：CC型个体日产奶量及乳糖、总固体含量均显著低于TT型个体，而SCS显著高于CT和TT型个体；该位点突变对乳中C14:1、C16:0、C16:1、C18:0、C18:3等5种脂肪酸含量和C14、C16和C18不饱和指数的影响均达到极显著水平(P<0.01)，TT基因型个体乳中C16:1含量、C16和C18不饱和指数极显著高于CC和CT型个体(P<0.01)。【结论】FADS2基因编码区c.908 C>T突变对中国荷斯坦牛泌乳性能和乳中脂肪酸组成有重要影响，TT基因型具有较高的日产奶量、总固体和C16:1含量及C16和C18不饱和指数。 |
| 关键词: FADS2基因 单核苷酸多态性 泌乳性能 脂肪酸组成 中国荷斯坦牛 |
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| 基金项目:国家自然科学基金项目（31372286，31272407，31472067）；江苏省研究生实践创新计划项目（SJZZ15_0182） |
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| Effects of FADS2 c.908 C>T mutation on milk production traits and fatty acids composition in milk of Chinese Holstein |
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WANG Mengqi,ZHU Xiaorui,XING Shiyu,et al
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| Abstract: |
| 【Objective】The objective of this study was to investigate the effects of FADS2 c.908 C>T mutation on milk production traits and fatty acids compositions in milk of Chinese Holstein.【Method】A total of 865 Chinese Holstein cows were genotyped using Sequenom MassARRAY (Sequenom Inc.,San Diego,CA) for the SNP mutation of FADS2 c.908 C>T,and the associations between SNPs and milk production traits,fatty acids composition,and unsaturated indexes of fatty acids in milk were analyzed by the least squares method.【Result】There were three genotypes (CC,CT and TT) of FADS2 c.908 C>T.CT was the dominant genotype,and allele C was dominant allele with the frequency of 0.592.The FADS2 c.908 C>T showed significant association with daily milk yield,fat percentage,protein percentage,total solid content,and SCS (P<0.05).The individuals with genotype CC had lower daily milk yield,lactose content,and total solid content than genotypes TT (P＜0.05),but higher SCS than genotypes CT and TT.The FADS2 c.908 C>T also showed very significant associations with percentages of C14:1,C16:0,C16:1,C18:0,C18:3 and unsaturated indexes of C14, C16 and C18 (P<0.01).The individuals with genotype TT had higher content of C16:1,and unsaturated indexes of C16 and C18 than genotypes CC and CT(P＜0.01).【Conclusion】The FADS2 c.908 C>T mutation had significant genetic effects on milk production traits and fatty acids composition in milk.The individuals with genotype CC had higher daily milk yield,total solid,content of C16:1,and unsaturated indexes of C16 and C18. |
| Key words: FADS2 gene single nucleotide polymorphism (SNP) milk production traits composition of fatty acids Chinese Holstein |
