| 摘要: |
| 【目的】正确评价茶树种质资源的遗传多样性，为有效保护和利用茶树种质资源奠定基础。【方法】利用10对STR引物，对来自中国华南、江南、江北和西南4大茶区的87份茶树种质，以及来自日本的“薮北种”及来源不详的“箫”茶树品种共计89份茶树种质进行遗传多样性和亲缘关系分析。【结果】10对STR引物共检测到124个等位位点，平均每个引物检测到等位位点12.4个，扩增位点期望杂合度的平均值高于观察杂合度的平均值。等位位点的观察杂合度平均为0.771 1，Shannon指数平均为1.978 0，多态信息含量平均为0.81。聚类结果表明，89份茶树种质资源遗传相似系数为0.77～0.98，平均为0.80，主要分成4个大类。【结论】89份茶树种质资源并未按地域严格分开，通过茶树种质资源所在不同茶区进行聚类分析发现，江北茶区茶资源与其他3大茶区亲缘关系较远。 |
| 关键词: 茶树 STR 遗传多样性 种质资源 茶区 |
| DOI： |
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| 基金项目:陕西省科技攻关项目（K333021407）；西北农林科技大学试验示范站(基地)科技创新与成果转化项目（NYY2013-77） |
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| Genetic diversity of 89 tea germplasm based on STR markers |
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LI Zhao,XIAO Bin,YU Youben,et al
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| Abstract: |
| 【Objective】This study evaluated diversity of tea germplasm resource to provide basis for protecting and using them.【Method】A total of 10 short tandom repeat (STR) markers were used to assess genetic diversity and relationship of 89 Camellia sinensis accessions,in which 87 were from Chinese tea areas (Huanan:south China,Jiangnan:areas south to the Yangtze River,Jiangbei:areas north to the Yangtze River,Xinan:southwest China),“Yabukita” was from Japan,and “Xiao” was unknown.【Result】A total of 124 putative alleles were detected from 10 primer pairs with a mean of 12.4 putative alleles per locus.The average value of expected heterozygosity was higher than the average of observed heterozygosity.The average observed heterozygosity,Shannon information index and polymorphism information content were 0.771 1,1.978 0 and 0.81,respectively.The Dice’s similarity coefficient between accessions ranged from 0.77 to 0.98 with a mean of 0.80.All accessions were divided into 4 groups.【Conclusion】The accessions were mainly divided into 4 groups but not according to origins.Tea accessions from area north to the Yangtze River were distant from the accessions from other areas. |
| Key words: tea STR genetic diversity germplasm tea area |
