| 摘要: |
| 【目的】以葵花籽仁为原料，优化提取绿原酸的工艺条件。【方法】以乙醇为提取溶剂，绿原酸提取率为指标，采用超声波微波辅助法提取葵花籽绿原酸，在单因素试验的基础上，选取液料比、超声波功率、微波功率为影响绿原酸提取率的主要因素，采用响应面试验方法对绿原酸提取工艺进行优化，并对绿原酸提取率的二次回归模型进行分析。【结果】单因素试验结果表明，绿原酸的提取率随着液料比的增加呈现先增大后保持不变的趋势，而随着乙醇体积分数、超声波功率、微波功率、微波辐射时间的增加呈现先增大后减小的变化趋势。响应面法优化的绿原酸最佳提取工艺条件为：液（mL）料(g)比25.47∶1，超声波功率307.1 W，微波功率539.36 W。经过修正得到的最佳工艺条件为：液料比25∶1，超声波功率300 W，微波功率540 W，乙醇体积分数65%，微波辐射时间90 s，此时绿原酸的提取率最高，可以达到3.27%。【结论】超声波微波辅助法提取葵花籽绿原酸具有操作简单、时间短、提取率高等特点。 |
| 关键词: 葵花籽 绿原酸 超声波 微波 响应面法 |
| DOI： |
| 分类号: |
| 基金项目:吉林省科技发展计划项目(2014011) |
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| Optimization of ultrasonic/microwave-assisted extraction of chlorogenic acid from sunflower seeds by response surface methodology |
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LIU Zhenchun,QIAN Yue
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| Abstract: |
| 【Objective】Optimum process conditions for extraction of chlorogenic acid from sunflower seed were determined.【Method】Using ethanol as extraction solvent and extraction yield of chlorogenic acid as index,ultrasonic/microwave-assisted method was used to extract chlorogenic acid from sunflower seed.On the basis of single factor experiments,ratio of liquor to material,ultrasonic power,and wave power were selected as main factors influencing the extraction yield.Then,response surface was used for process optimization,and the extraction yield of chlorogenic acid was analyzed using quadratic regression model.【Result】Experimental results of single factors showed that the extraction yield of chlorogenic acid increased firstly and remained subsequently with the increase of ratio of liquor to material,while it increased firstly and dropped subsequently with the increase of ethanol volume fraction,ultrasonic power,wave power and microwave time.Optimum conditions of the extraction of chlorogenic acid using response surface method were:ratio of liquor (mL) to material (g) 25.47∶1,ultrasonic power 307.1 W,and microwave power 539.36 W.The revised best process conditions were:ratio of liquor to material 25∶1,ultrasonic power 300 W,microwave power 540 W,ethanol volume fraction 65%,and microwave time 90s,under which the highest extraction yield of chlorogenic acid was 3.27%.【Conclusion】The ultrasonic/microwave-assisted extraction method of chlorogenic acid from sunflower seeds had the advantages of simple operation,short time and high extraction yield. |
| Key words: sunflower seeds chlorogenic acid ultrasonic microwave response surface methodology |
