| 摘要: |
| 【目的】分离、鉴定三七锈腐病的病原菌，为三七锈腐病的防治提供参考。【方法】从云南省文山州砚山县盘龙乡文山三七科技示范园中采集病株标样，采用柯赫氏法则确定三七锈腐病的病原菌，通过形态学和分子生物学方法鉴定病原菌；以马铃薯蔗糖培养基（PSA）、马铃薯葡萄糖培养基（PDA）、察氏培养基（Czapek’s）和胡萝卜琼脂培养基(CA)为基础培养基，研究20种不同配方条件下三七锈腐病菌的产孢情况。【结果】从三七锈腐病根部分离得到8个单孢菌株，其中4株具有致病性，选取致病性最强的菌株Cd-M-6进行形态学和分子生物学鉴定，结果表明，毁灭柱孢Cylindrocarpon destructans(Zins.) Scholten是引起三七锈腐病的病原菌。在以PSA和PDA为基础培养基的配方中，随着马铃薯用量的增加，三七锈腐病菌的产孢量逐渐增加；当马铃薯用量为200 g/L时，三七锈腐病菌在PSA上的产孢量最大；在PDA培养基上，当马铃薯用量为150 g/L时，较适合三七锈腐病菌产孢。在Czapek’s培养基上，蔗糖用量为30 g/L时，三七锈腐病菌的产孢量最大；在CA培养基上，胡萝卜用量为400 g/L时，三七锈腐病菌的产孢量最大。PSA、PDA、Czapek’s、CA 4种培养基相比，三七锈腐病菌在PSA上的产孢量最大。【结论】毁灭柱孢C.destructans是三七锈腐病的病原菌；在供试培养基中，PSA是三七锈腐病菌的最佳产孢培养基。 |
| 关键词: 三七 锈腐病 病原菌鉴定 产孢培养基 |
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| 基金项目:云南省省院省校合作项目（2012IB023） |
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| Characterization of Sanqi (Panax notoginseng) rust pathogen and selection of optimal conidia production medium |
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MAO Zhong-shun,LONG Yue-juan,ZHU Shu-sheng,et al
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| Abstract: |
| 【Objective】Rusty rot has the highest incidence and the largest harm among Sanqi (Panax notoginseng) root rots.This study focused on the isolation and characterization of pathogen of Sanqi rusty rot to provide reference for the prevention and control in practice.【Method】The samples were collected from Wenshan Sanqi technology demonstration park in Panlong Town,Yanshan County,Wenshan,Yunnan.Isolates from samples were determined by Koch’s postulate,and characterized morphologically and molecularly.Twenty recipes were made based on potato saccharose agar (PSA),potato dextrose agar (PDA),Czapek’s,and carrot agar (CA) medium to test the sporulation of the pathogen of Sanqi rusty rot.【Result】Four out of eight obtained single-spore isolates of Sanqi rusty rot pathogen were aggressive.The most aggressive isolate Cd-M-6 was selected for morphological and molecular characterization.Isolate Cd-M-6 was identified as Cylindorcarpon destructans (Zins.) Scholten.The sporulation of C.destructans increased with the increase of potato weight on PSA and PDA plates.The maximum sporulation was obtained when the weight of potato was 200 g/L on PSA,and 150 g/L was proper for sporulation of C.destructans on PDA.30 g/L of saccharose was the most proper threshold for sporulation of C.destructans on Czapek’s plate.The sporulation of C.destructans was the maximum when 400 g/L of carrot was used on CA plate.The sporulation of C.destructans on PSA was the largest compared to other media.【Conclusion】Cylindorcarpon destructans was the pathogen of Sanqi rusty rot.The sporulation of C.destructans was the maximum on PSA. |
| Key words: Sanqi(Panax notoginseng) rusty rot pathogen identification conidium production media |
