| 摘要: |
| 【目的】研究水杨酸（SA）对华北落叶松种子萌发和幼苗生长的影响，为水杨酸在华北落叶松育苗中的科学应用提供理论依据。【方法】采用SA浸种和外施2种方式，设置0.1，0.5和1.0 mmol/L 3个SA浓度处理华北落叶松种子,以蒸馏水处理为对照，在25 ℃培养条件下分析SA对华北落叶松种子萌发和幼苗SOD、POD活性及MDA、可溶性糖含量的影响，并比较培养温度为25，30和35 ℃时，各处理幼苗POD、SOD活性以及MDA和可溶性糖含量的差异。【结果】SA浸种可以显著提高华北落叶松种子的发芽率，以0.1 mmol/L处理最高，发芽率为87.22%，0.5 mmol/L处理次之，对照发芽率仅为54.02%；0.1 mmol/L SA处理的胚根长度较对照提高83.91%，胚芽长提高84.67%，0.5 mmol/L处理次之，但对胚芽长的提高作用不显著；0.1 mmol/L SA浸种处理在30和35 ℃下的POD活性分别较对照提高 3.59%和174.42%，SOD活性分别较对照提高86.98%和109.82%，而外施SA处理幼苗的POD和SOD活性在30 ℃下分别较对照提高217.82%和128.15%，35 ℃条件下分别提高56.77%和277.67%；30 和35 ℃条件下，0.1 mmol/L SA浸种处理幼苗的MDA含量分别较对照降低17.72%和7.32%，外施SA处理分别降低12.94%和11.10%； 0.1和0.5 mmol/L SA处理幼苗的可溶性糖含量均显著高于对照。【结论】在SA处理后的12 d内，0.1～0.5 mmol/L的SA对促进华北落叶松胚根、胚芽生长和提高幼苗SOD、POD酶活性及降低MDA含量效果较佳。 |
| 关键词: 水杨酸 华北落叶松 种子萌发 POD SOD MDA |
| DOI： |
| 分类号: |
| 基金项目:山西省自然科学基金项目（2012011033-4） |
|
| Effect of salicylic acid on seed germination and seedling physiological characteristics of Larix principis-rupprechtii Mayrn |
|
FAN Xiao-long,TAO Li,MA Guo-qiang,et al
|
| Abstract: |
| 【Objective】This study investigated the effect of salicylic acid (SA) on Larix principis-rupprechtii seed germination and seedling growth to improve the application of SA in Larix principis-rupprechtii nursery.【Method】Soaking and exogenous applications of SA were used to process Larix principis-rupprechtii seed with 3 concentrations (0.1,0.5,1.0 mmol/L).Water was used as control and temperature was 25 ℃.The effects on seed germination,activities of POD and SOD and contents of MDA and soluble sugar of each treatment were analyzed and their differences at temperatures of 25,30,and 35 ℃ were compared.【Result】SA soaking significantly improved the germination rate of Larix principis-rupprechtii.0.1 mmol/L treatment showed the highest rate of 87.22%,followed by 0.5 mmol/L treatment,the control only 54.02%.The radicle and germ lengths were increased by 83.91% and 84.67% with 0.1 mmol/L SA followed by 0.5 mmol/L.0.1 mmol/L SA seed soaking improved POD and SOD activities by 3.59% and 86.98% at 30 ℃ and by 174.42% and 109.82% at 35 ℃,while exogenous application of SA improved POD and SOD activities by 217.82% and 128.15% at 30 ℃ and by 56.77% and 277.67% at 35 ℃ followed by 0.5 mmol/L treatment.Soaking treatment decreased MDA content by 17.72% and 7.32% while exogenous SA treatment decreased MDA content by 12.94% and 11.10% at 30 and 35 ℃,respectively.Soluble sugar contents treated with 0.1 and 0.5 mmol/L SA were significantly higher than the control.【Conclusion】Within 12 days of seed treatment,0.1－0.5 mmol/L SA was better to improve the growth of radicle and germ,increase SOD and POD activities and decrease MDA content. |
| Key words: salicylic acid Larix principis-rupprechtii Mayrn seed germination POD SOD MDA |
